• Western blot analysis using Elk1 monoclonal antibody against truncated ELK1 recombinant protein (1) and K562 cell lysate (2).

Anti-Elk1 antibody (STJ98018)

SKU:
STJ98018
Current Stock:
Host: Mouse
Applications: WB, ELISA
Reactivity: Human
Short Description: Mouse monoclonal primary antibody against Elk1.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 7E10D5/7E10E8
Conjugation: Unconjugated
Formulation: Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.
Purification: Affinity purification
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/mL
Isotype: IgG1
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Specificity: Elk1 monoclonal antibody detects endogenous levels of Elk1 protein.
Uniprot ID: ELK1_HUMAN
Gene ID: 2002
Gene Symbol: ELK1
Immunogen: Purified recombinant fragment of Elk1 expressed in E. Coli.
Tissue Specificity Lung and testis.
Post Translational Modifications Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention. On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1.
Database Links Reactome: R-HSA-198753
Cellular Localisation Nucleus
Protein Name Ets Domain-Containing Protein Elk-1
Alternative Names Anti-Ets Domain-Containing Protein Elk-1 antibody
Anti-ELK1 antibody

Information sourced from Uniprot.org

6 months standard