| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DNA-methyltransferase 3A (10-90 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DNMT3A |
| Gene ID: |
1788 |
| Uniprot ID: |
DNM3A_HUMAN |
| Immunogen Region: |
10-90 aa |
| Specificity: |
DNM3A Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: |
Synthesized peptide derived from the human protein at the amino acid range 10-90 |
| Post Translational Modifications | Sumoylated.sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. Auto-methylated at Cys-710: auto-methylation takes place in absence of DNA substrate and inactivates the DNA methyltransferase activity. Inactivation by auto-methylation may be used to inactivate unused DNA methyltransferases in the cell. |
| Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Also has weak auto-methylation activity on Cys-710 in absence of DNA. |
| Protein Name | DnaCytosine-5-Methyltransferase 3aDnmt3aCysteine Methyltransferase Dnmt3aDna Methyltransferase HsaiiiaDna Mtase HsaiiiaM.hsaiiia |
| Database Links | Reactome: R-HSA-212300Reactome: R-HSA-3214858Reactome: R-HSA-4655427Reactome: R-HSA-5334118Reactome: R-HSA-9710421 |
| Cellular Localisation | NucleusChromosomeCytoplasmAccumulates In The Major Satellite Repeats At Pericentric Heterochromatin |
| Alternative Antibody Names | Anti-Dna antibodyAnti-Cytosine-5-Methyltransferase 3a antibodyAnti-Dnmt3a antibodyAnti-Cysteine Methyltransferase Dnmt3a antibodyAnti-Dna Methyltransferase Hsaiiia antibodyAnti-Dna Mtase Hsaiiia antibodyAnti-M.hsaiiia antibodyAnti-DNMT3A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
