• Western blot analysis of extracts of various cell lines, using DDX39A antibody (STJ114432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DDX39A antibody (178-427) (STJ114432)

SKU:
STJ114432

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDX39A (178-427) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX39A
Gene ID: 10212
Uniprot ID: DX39A_HUMAN
Immunogen Region: 178-427
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 178-427 of human DDX39A (NP_005795.2).
Immunogen Sequence: LVRNRSFSLKNVKHFVLDEC DKMLEQLDMRRDVQEIFRLT PHEKQCMMFSATLSKDIRPV CRKFMQDPMEVFVDDETKLT LHGLQQYYVKLKDSEKNRKL FDLLDVLEFNQVIIFVKSVQ RCMALAQLLVEQNFPAIAIH RGMAQEERLSRYQQFKDFQR RILVATNLFGRGMDIERVNI VFNYDMPEDSDTYLHRVARA GRFGTKGLAITFVSDENDAK ILNDVQDRFEVNVAELPEE
Tissue Specificity Detected in testis, and at lower levels in brain, kidney, lung, thymus, spleen and salivary gland.
Function Isoform 1: Involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus.
Protein Name Atp-Dependent Rna Helicase Ddx39a
Dead Box Protein 39
Nuclear Rna Helicase Urh49
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72187
Reactome: R-HSA-73856
Cellular Localisation Nucleus
Cytoplasm
Can Translocate To The Cytoplasm In The Presence Of Mx1
Alternative Antibody Names Anti-Atp-Dependent Rna Helicase Ddx39a antibody
Anti-Dead Box Protein 39 antibody
Anti-Nuclear Rna Helicase Urh49 antibody
Anti-DDX39A antibody
Anti-DDX39 antibody

Information sourced from Uniprot.org

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