• Western blot analysis of extracts of various cell lines, using CYP2A6 Antibody (STJ28378) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-CYP2A6 antibody (STJ28378)

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STJ28378
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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal primary antibody against CYP2A6.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: CP2A6_HUMAN
Gene ID: 1548
Gene Symbol: CYP2A6
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 215-494 of human CYP2A6 (NP_000753.3).
Background This gene, CYP2A6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin, and also metabolizes nicotine, aflatoxin B1, nitrosamines, and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype, meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6.
Tissue Specificity Liver.
Function Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4-cineole 2-exo-monooxygenase. Possesses low phenacetin O-deethylation activity.
Database Links Reactome: R-HSA-211981
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Microsome Membrane
Protein Name Cytochrome P450 2a6
1 -4-Cineole 2-Exo-Monooxygenase
Cypiia6
Coumarin 7-Hydroxylase
Cytochrome P450 Iia3
Cytochrome P450(I
Alternative Names Anti-Cytochrome P450 2a6 antibody
Anti-1 -4-Cineole 2-Exo-Monooxygenase antibody
Anti-Cypiia6 antibody
Anti-Coumarin 7-Hydroxylase antibody
Anti-Cytochrome P450 Iia3 antibody
Anti-Cytochrome P450(I antibody
Anti-CYP2A6 antibody
Anti-CYP2A3 antibody

Information sourced from uniprot.org

6 months standard