• Immunofluorescence analysis of U2OS cells using CXCR5 antibody (STJ29912) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CXCR5 antibody (STJ29912) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

Anti-CXCR5 antibody (STJ29912)

SKU:
STJ29912
Current Stock:
Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal primary antibody against CXCR5.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IF: 1:50-1:200
Uniprot ID: CXCR5_HUMAN
Gene ID: 643
Gene Symbol: CXCR5
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human CXCR5 (NP_001707.1).
Background This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC) , and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Tissue Specificity Expression in mature B-cells and Burkitt lymphoma cells.
Function Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
Database Links Reactome: R-HSA-380108
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Protein Name C-X-C Chemokine Receptor Type 5
Cxc-R5
Cxcr-5
Burkitt Lymphoma Receptor 1
Monocyte-Derived Receptor 15
Mdr-15
Cd Antigen Cd185
Alternative Names Anti-C-X-C Chemokine Receptor Type 5 antibody
Anti-Cxc-R5 antibody
Anti-Cxcr-5 antibody
Anti-Burkitt Lymphoma Receptor 1 antibody
Anti-Monocyte-Derived Receptor 15 antibody
Anti-Mdr-15 antibody
Anti-Cd Antigen Cd185 antibody
Anti-CXCR5 antibody
Anti-BLR1 antibody
Anti-MDR15 antibody
6 months standard