• Western blot analysis of extracts of various cell lines, using CXCR5 rabbit monoclonal antibody (STJ11102401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Confocal imaging of human tonsil using CXCR5 Rabbit monoclonal antibody (STJ11102401, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.
  • Immunofluorescence analysis of rat spleen using CXCR5 rabbit monoclonal antibody (STJ11102401) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CXCR5 Rabbit monoclonal antibody (STJ11102401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of mouse spleen using CXCR5 rabbit monoclonal antibody (STJ11102401) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CXCR5 antibody (273-372) [S1MR] (STJ11102401)

SKU:
STJ11102401

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CXCR5 (273-372) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CXCR5
Gene ID: 643
Uniprot ID: CXCR5_HUMAN
Immunogen Region: 273-372
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 273-372 of human CXCR5 (P32302).
Immunogen Sequence: SPYHIVIFLDTLARLKAVDN TCKLNGSLPVAITMCEFLGL AHCCLNPMLYTFAGVKFRSD LSRLLTKLGCTGPASLCQLF PSWRRSSLSESENATSLTTF
Tissue Specificity Expression in mature B-cells and Burkitt lymphoma cells.
Function Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
Protein Name C-X-C Chemokine Receptor Type 5
Cxc-R5
Cxcr-5
Burkitt Lymphoma Receptor 1
Monocyte-Derived Receptor 15
Mdr-15
Cd Antigen Cd185
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-C-X-C Chemokine Receptor Type 5 antibody
Anti-Cxc-R5 antibody
Anti-Cxcr-5 antibody
Anti-Burkitt Lymphoma Receptor 1 antibody
Anti-Monocyte-Derived Receptor 15 antibody
Anti-Mdr-15 antibody
Anti-Cd Antigen Cd185 antibody
Anti-CXCR5 antibody
Anti-BLR1 antibody
Anti-MDR15 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance