||Human, Mouse, Rat, Dog, Bovine
||Goat polyclonal anti-Cortactin/EMS1 antibody is suitable for use in ELISA and Western Blot.
||FOR RESEARCH USE ONLY (RUO).
||Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at-20°C. Minimize freezing and thawing.
||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
||Store at-20°C and avoid freeze-thaw cycles.
||WB-0.01-0.03µg/mlELISA-antibody detection limit dilution 1:32000.
||This antibody is expected to recognize both reported isoforms (NP_005222.2; NP_612632.1).
| Post Translational Modifications || Phosphorylated by FER. Phosphorylated in response to FGR activation. Phosphorylation by SRC promotes MYLK binding. Phosphorylated on tyrosine residues in response to CHRM1 activation. Phosphorylated by PTK2/FAK1 in response to cell adhesion. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. Phosphorylated by PKN2 at both serine and threonine residues in a GTP-bound Rac1-dependent manner in hyaluronan-induced astrocytes and hence down-regulated CTTN ability to associate with filamentous actin. |
| Function || Contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density. Plays a role in the invasiveness of cancer cells, and the formation of metastases. Plays a role in focal adhesion assembly and turnover. In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits. Required for stabilization of KCNH1 channels at the cell membrane. |
| Protein Name || Src Substrate Cortactin |
| Database Links || Reactome: R-MMU-8856828 |
| Cellular Localisation || CytoplasmCytoskeletonCell ProjectionLamellipodiumRuffleCell CortexPodosomeDendritic SpineDendriteCell MembranePeripheral Membrane ProteinCytoplasmic SideCell JunctionMembraneClathrin-Coated PitFocal AdhesionColocalizes Transiently With Ptk2/Fak1 At Focal AdhesionsAssociated With Membrane Ruffles And LamellipodiaIn The Presence Of Cttnbp2nlColocalizes With Stress FibersIn The Presence Of Cttnbp2Localizes At The Cell CortexIn Response To Neuronal Activation By GlutamateRedistributes From Dendritic Spines To The Dendritic ShaftColocalizes With Dnm2 At The Basis Of Filopodia In Hippocampus Neuron Growth Zones |
| Alternative Antibody Names || Anti-Src Substrate Cortactin antibodyAnti-Cttn antibodyAnti-Ems1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance