• STJ70093 (0.1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
  • STJ70093 (0.01µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70093 (0.03µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
  • STJ70093 (0.1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
  • STJ70093 (0.01µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70093 (0.03µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
  • STJ70093 (0.1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
  • STJ70093 (0.01µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70093 (0.03µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-Cortactin/EMS1 Antibody (STJ70093)

SKU:
STJ70093

Current Stock:
Host: Goat
Applications: Pep-ELISA, WB
Reactivity: Human, Mouse, Rat, Dog, Bovine
Short Description: Goat polyclonal anti-Cortactin/EMS1 antibody is suitable for use in ELISA and Western Blot.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage Instruction: Store at-20°C and avoid freeze-thaw cycles.
Concentration: 0.5mg/mL
Isotype: IgG
Dilution Range: WB-0.01-0.03µg/ml
ELISA-antibody detection limit dilution 1:32000.
Specificity: This antibody is expected to recognize both reported isoforms (NP_005222.2; NP_612632.1).
Uniprot ID: SRC8_MOUSE
Gene ID: 13043
Gene Symbol: Cttn
Immunogen Region: N Terminus
Immunogen Sequence: WKASAGHAVSIA
Accession Number: NP_005222.2; NP_612632.1
Post Translational Modifications Phosphorylated by FER. Phosphorylated in response to FGR activation. Phosphorylation by SRC promotes MYLK binding. Phosphorylated on tyrosine residues in response to CHRM1 activation. Phosphorylated by PTK2/FAK1 in response to cell adhesion. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. Phosphorylated by PKN2 at both serine and threonine residues in a GTP-bound Rac1-dependent manner in hyaluronan-induced astrocytes and hence down-regulated CTTN ability to associate with filamentous actin.
Function Contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density. Plays a role in the invasiveness of cancer cells, and the formation of metastases. Plays a role in focal adhesion assembly and turnover. In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits. Required for stabilization of KCNH1 channels at the cell membrane.
Protein Name Src Substrate Cortactin
Database Links Reactome: R-MMU-8856828
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Lamellipodium
Ruffle
Cell Cortex
Podosome
Dendritic Spine
Dendrite
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Junction
Membrane
Clathrin-Coated Pit
Focal Adhesion
Colocalizes Transiently With Ptk2/Fak1 At Focal Adhesions
Associated With Membrane Ruffles And Lamellipodia
In The Presence Of Cttnbp2nl
Colocalizes With Stress Fibers
In The Presence Of Cttnbp2
Localizes At The Cell Cortex
In Response To Neuronal Activation By Glutamate
Redistributes From Dendritic Spines To The Dendritic Shaft
Colocalizes With Dnm2 At The Basis Of Filopodia In Hippocampus Neuron Growth Zones
Alternative Antibody Names Anti-Src Substrate Cortactin antibody
Anti-Cttn antibody
Anti-Ems1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance