• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using IL-1 beta (Cleaved-Asp210) Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Cleaved-CASP1-M211 antibody (192-241 aa) (STJ90002)

SKU:
STJ90002

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cleaved-Caspase-1-M211 (192-241 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CASP1
Gene ID: 834
Uniprot ID: CASP1_HUMAN
Immunogen Region: 192-241 aa
Specificity: Cleaved-Caspase-1 (M211) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-1 protein resulting from cleavage adjacent to M211.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CASP1 at the amino acid range 192-241
Post Translational Modifications The two subunits are derived from the precursor sequence by an autocatalytic mechanism. Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8. Cleavage in the interdomain linker region is required to induce pyroptosis.
Function Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly. Isoform Delta: Apoptosis inactive. Isoform Epsilon: Apoptosis inactive.
Protein Name Caspase-1
Casp-1
Interleukin-1 Beta Convertase
Il-1bc
Interleukin-1 Beta-Converting Enzyme
Ice
Il-1 Beta-Converting Enzyme
P45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-448706
Reactome: R-HSA-5620971
Reactome: R-HSA-6803207
Reactome: R-HSA-844456
Reactome: R-HSA-844615
Reactome: R-HSA-844623
Reactome: R-HSA-9008059
Reactome: R-HSA-9660826
Reactome: R-HSA-9692916
Cellular Localisation Cytoplasm
Cell Membrane
Alternative Antibody Names Anti-Caspase-1 antibody
Anti-Casp-1 antibody
Anti-Interleukin-1 Beta Convertase antibody
Anti-Il-1bc antibody
Anti-Interleukin-1 Beta-Converting Enzyme antibody
Anti-Ice antibody
Anti-Il-1 Beta-Converting Enzyme antibody
Anti-P45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 antibody
Anti-CASP1 antibody
Anti-IL1BC antibody
Anti-IL1BCE antibody

Information sourced from Uniprot.org

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