Anti-CEP164 antibody (650-700 aa) (STJ13100992)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 650-700 of human CEP164 conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-CEP164 (650-700 aa) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | CEP164 |
| Gene ID | 22897 |
| Uniprot ID | CE164_HUMAN |
| Immunogen | A synthetic peptide from aa region 650-700 of human CEP164 conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | 650-700 aa |
| Specificity | Specific for CEP164. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-186 is induced upon DNA-damage caused by treatment with IR irradiation, UV irradiation, hydroxyurea or amphidicolin. Also MDC1-mediated chromatin remodeling is critical for DNA damage-induced phosphorylation. |
| Function | Plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1. |
| Protein Name | Centrosomal Protein Of 164 KdaCep164 |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-8854518 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleNucleusLocalizes Specifically To Very Distally Located Appendage Structures On The Mature Centriole From Which Initiate Pc FormationPersisted At Centrioles Throughout MitosisExpressed In Chromatin-Enriched Nuclear Fraction Of Hela CellsIn Response To Dna DamageIt Translocates To Nuclear Foci That Contain The Dna Damage Response Proteins Kat5/Tip60 And Chek1 |
| Alternative Antibody Names | Anti-Centrosomal Protein Of 164 Kda antibodyAnti-Cep164 antibodyAnti-CEP164 antibodyAnti-KIAA1052 antibodyAnti-NPHP15 antibody |
Information sourced from Uniprot.org