• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Centromeric Protein A Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HepG2 cells, using Centromeric Protein A Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CENPA antibody (1-50 aa) (STJ92218)

SKU:
STJ92218

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Histone H3-like centromeric protein A (1-50 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CENPA
Gene ID: 1058
Uniprot ID: CENPA_HUMAN
Immunogen Region: 1-50 aa
Specificity: CENP-A Polyclonal Antibody detects endogenous levels of CENP-A protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Centromeric Protein A at the amino acid range 1-50
Function Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin that serves as an assembly site for the inner kinetochore. The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis.
Protein Name Histone H3-Like Centromeric Protein A
Centromere Autoantigen A
Centromere Protein A
Cenp-A
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-606279
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Localizes Exclusively To Sites Of Kinetochore Assembly In Centromeres
Occupies A Compact Domain At The Inner Kinetochore Plate Stretching Across 2 Thirds Of The Length Of The Constriction But Encompassing Only One Third Of The Constriction Width And Height
Phosphorylation At Ser-68 During Early Mitosis Abolishes Association With Chromatin And Centromeres And Results In Dispersed Nuclear Location
Alternative Antibody Names Anti-Histone H3-Like Centromeric Protein A antibody
Anti-Centromere Autoantigen A antibody
Anti-Centromere Protein A antibody
Anti-Cenp-A antibody
Anti-CENPA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance