• Formalin-fixed, paraffin-embedded human pleomorphic liposarcoma stained with anti-CDK4 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear/cytplasmic staining of tumor cells

Anti-CDK4 antibody (STJ180074)

SKU:
STJ180074
Current Stock:
Host: Mouse
Applications: IHC-p
Reactivity: Human
Short Description: Mouse monoclonal primary antibody against CDK4.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: DCS‐31
Conjugation: Unconjugated
Formulation: Purified antibody diluted in Tris‐HCI buffer containing stabilizing protein and <0.1% sodium azide.
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Isotype: IgG2 Alpha
Dilution Range: 1:50‐100
Uniprot ID: CDK4_HUMAN
Gene ID: 1019
Gene Symbol: CDK4
Immunogen: Full length CDK4 of human origin.
Immunogen Region: Full length
Background CDK4 is a major catalytic subunit of mammalian D‐type cyclins. The gene for cdk4 is localized to human chromosome 12q13. The cdk4 protein is constitutively expressed throughout the cell cycle and it is the assembly and accumulation of functional cdk4/cyclin D complexes which is rate limiting for the progression through the G1 phase of the cell cycle. Once the complex is formed it must be activated by the phosphorylation of threonine 172 on the cdk4 subunit, a reaction mediated by a cdk‐activating kinase (CAK). Complex formation and phosphorylation are independently regulated events. The phosphorylation of the cdk4 subunit is subject to regulation of both stimulatory and inhibitory cytokine signals thereby allowing cdk4 to function as a mid G1 checkpoint. CDK4 gene is amplified in well differentiated liposarcoma.
Post Translational Modifications Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
Function Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Database Links Reactome: R-HSA-187577
Cellular Localisation Cytoplasm
Nucleus
Nucleus Membrane
Cytoplasmic When Non-Complexed
Forms A Cyclin D-Cdk4 Complex In The Cytoplasm As Cells Progress Through G(1) Phase
The Complex Accumulates On The Nuclear Membrane And Enters The Nucleus On Transition From G(1) To S Phase
Also Present In Nucleoli And Heterochromatin Lumps
Colocalizes With Rb1 After Release Into The Nucleus
Protein Name Cyclin-Dependent Kinase 4
Cell Division Protein Kinase 4
Psk-J3
Alternative Names Anti-Cyclin-Dependent Kinase 4 antibody
Anti-Cell Division Protein Kinase 4 antibody
Anti-Psk-J3 antibody
Anti-CDK4 antibody
Positive Control Well‐differentiated liposarcoma.
6 months standard