• STJ70446 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
  • STJ70446 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
  • STJ70446 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
  • STJ70446 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
  • STJ70446 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
  • STJ70446 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo

Anti-CDEP/FARP1 Antibody (STJ70446)

SKU:
STJ70446

Current Stock:
Host: Goat
Applications: Pep-ELISA, IF, FC
Reactivity: Human, Mouse, Rat, Bovine
Short Description: Goat polyclonal anti-CDEP/FARP1 antibody is suitable for use in ELISA, Immunofluorescence and Flow Cytometry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage Instruction: Store at-20°C and avoid freeze-thaw cycles.
Concentration: 0.5mg/mL
Isotype: IgG
Dilution Range: WB-0.3-1µg/ml
IF-Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. 10µg/ml
FC-Flow cytometric analysis of HepG2 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:32000.
Uniprot ID: FARP1_HUMAN
Gene ID: 10160
Gene Symbol: FARP1
Immunogen Region: N Terminus
Immunogen Sequence: GEIEQRPTPGSRL
Accession Number: NP_005757.1; NP_001001715.2; NP_001273768.1
Function Functions as guanine nucleotide exchange factor for RAC1. May play a role in semaphorin signaling. Plays a role in the assembly and disassembly of dendritic filopodia, the formation of dendritic spines, regulation of dendrite length and ultimately the formation of synapses.
Protein Name Ferm - Arhgef And Pleckstrin Domain-Containing Protein 1
Chondrocyte-Derived Ezrin-Like Protein
Ferm - Rhogef And Pleckstrin Domain-Containing Protein 1
Pleckstrin Homology Domain-Containing Family C Member 2
Ph Domain-Containing Family C Member 2
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Junction
Synapse
Synaptosome
Cytoplasm
Cytosol
Cell Projection
Filopodium
Dendrite
Dendritic Spine
Recruited To The Cell Membrane Via Interaction With Cadm1
Alternative Antibody Names Anti-Ferm - Arhgef And Pleckstrin Domain-Containing Protein 1 antibody
Anti-Chondrocyte-Derived Ezrin-Like Protein antibody
Anti-Ferm - Rhogef And Pleckstrin Domain-Containing Protein 1 antibody
Anti-Pleckstrin Homology Domain-Containing Family C Member 2 antibody
Anti-Ph Domain-Containing Family C Member 2 antibody
Anti-FARP1 antibody
Anti-CDEP antibody
Anti-PLEKHC2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance