| Host: | Rabbit |
| Applications: | WB/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-CDC27 (551-830) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDC27 |
| Gene ID: | 996 |
| Uniprot ID: | CDC27_HUMAN |
| Immunogen Region: | 551-830 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 551-830 of human CDC27 (NP_001107563.1). |
| Immunogen Sequence: | HLQKDVALSVLSKDLTDMDK NSPEAWCAAGNCFSLQREHD IAIKFFQRAIQVDPNYAYAY TLLGHEFVLTEELDKALACF RNAIRVNPRHYNAWYGLGMI YYKQEKFSLAEMHFQKALDI NPQSSVLLCHIGVVQHALKK SEKALDTLNKAIVIDPKNPL CKFHRASVLFANEKYKSALQ ELEELKQIVPKESLVYFLIG KVYKKLGQTHLALMNFSWAM DLDPKGANNQIKEAIDKRY |
| Post Translational Modifications | Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis. |
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. |
| Protein Name | Cell Division Cycle Protein 27 HomologAnaphase-Promoting Complex Subunit 3Apc3Cdc27 HomologCdc27hsH-Nuc |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-174048Reactome: R-HSA-174084Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-174184Reactome: R-HSA-176407Reactome: R-HSA-176408Reactome: R-HSA-176409Reactome: R-HSA-176412Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2559582Reactome: R-HSA-68867Reactome: R-HSA-69017Reactome: R-HSA-8853884Reactome: R-HSA-9687136Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindle |
| Alternative Antibody Names | Anti-Cell Division Cycle Protein 27 Homolog antibodyAnti-Anaphase-Promoting Complex Subunit 3 antibodyAnti-Apc3 antibodyAnti-Cdc27 Homolog antibodyAnti-Cdc27hs antibodyAnti-H-Nuc antibodyAnti-CDC27 antibodyAnti-ANAPC3 antibodyAnti-D0S1430E antibodyAnti-D17S978E antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
