![Anti-CD28 nanobody [SAA1283] (STJN000135)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/200748/412930/STJN000135-SDS-PAGE-1__08243.1749232463.jpg?c=1 "Anti-CD28 nanobody [SAA1283] (STJN000135)")
![Anti-CD28 nanobody [SAA1283] (STJN000135)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/200748/412930/STJN000135-SDS-PAGE-1__08243.1749232463.jpg?c=1 "Anti-CD28 nanobody [SAA1283] (STJN000135)")
Anti-CD28 nanobody [SAA1283] (STJN000135)
SPECIFICATIONS
ClonalityMonoclonal
HostAlpaca
ConjugationUnconjugated
IsotypeVHH-8His-Cys-tag
General Information
| Short Description | Alpaca monoclonal nanobody anti-T-Cell-Specific Surface Glycoprotein Cd28 is suitable for use in ELISA, Flow Cytometry and SPR research applications. |
| Applications | ELISA/FC/SPR |
| Host | Alpaca |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA1283 |
| Isotype | VHH-8His-Cys-tag |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by Nickel column. |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at-20°C 12 months. Store at-80°C long term. |
Target Information
| Gene Symbol | CD28 |
| Gene ID | 940 |
| Uniprot ID | CD28_HUMAN |
Additional Info
| Post Translational Modifications | Phosphorylated by LCK. Dephosphorylated by PTPN11. Isoform 3: Tyrosine phosphorylated induced by CD40LG. |
| Function | Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells. Isoform 3: Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. |
| Protein Name | T-Cell-Specific Surface Glycoprotein Cd28Tp44Cd Antigen Cd28 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-164939Reactome: R-HSA-2219530Reactome: R-HSA-389356Reactome: R-HSA-389357Reactome: R-HSA-389359Reactome: R-HSA-6811558 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 3: Cell Surface |
| Alternative Nanobody Names | Anti-T-Cell-Specific Surface Glycoprotein Cd28 nanobodyAnti-Tp44 nanobodyAnti-Cd Antigen Cd28 nanobodyAnti-CD28 nanobody |
Information sourced from Uniprot.org