![Anti-CD223/LAG3 nanobody [Clone:3132] (STJN000590)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/201203/413301/STJN000590-SDS-PAGE-1__97537.1749232778.jpg?c=1 "Anti-CD223/LAG3 nanobody [Clone:3132] (STJN000590)")
![Anti-CD223/LAG3 nanobody [Clone:3132] (STJN000590)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/201203/413301/STJN000590-SDS-PAGE-1__97537.1749232778.jpg?c=1 "Anti-CD223/LAG3 nanobody [Clone:3132] (STJN000590)")
Anti-CD223/LAG3 nanobody [Clone:3132] (STJN000590)
SPECIFICATIONS
ClonalityMonoclonal
HostAlpaca
ConjugationUnconjugated
IsotypeVHH-8His-Cys-tag
General Information
| Short Description | Alpaca monoclonal nanobody anti-CD223/LAG3 is suitable for use in ELISA and FuncS research applications. |
| Applications | ELISA/FuncS |
| Host | Alpaca |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | Clone:3132 |
| Isotype | VHH-8His-Cys-tag |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by Nickel column. |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at-20°C 12 months. Store at-80°C long term. |
Target Information
| Gene Symbol | Lag3 |
| Gene ID | 16768 |
| Uniprot ID | LAG3_MOUSE |
Additional Info
| Post Translational Modifications | Lymphocyte activation gene 3 protein: Proteolytically cleaved by ADAM10 and ADAM17 within the connecting peptide region, leading to release of Secreted lymphocyte activation gene 3 protein (sLAG-3). ADAM10 mediates constitutive cleavage, but cleavage increases following T-cell activation, whereas shedding by ADAM17 is induced by TCR signaling in a PRKCQ-dependent manner. |
| Function | Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as FGL1. FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function. Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1. Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells. Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function. Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation. Binds MHC class II (MHC-II).the precise role of MHC-II-binding is however unclear. Secreted lymphocyte activation gene 3 protein: May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response. |
| Protein Name | Lymphocyte Activation Gene 3 ProteinLag-3Activation-Induced Cytidine Deaminase-Linked Autoimmunity ProteinAidaCd Antigen Cd223 Cleaved Into - Secreted Lymphocyte Activation Gene 3 ProteinSlag-3 |
| Database Links | Reactome: R-MMU-2132295 |
| Cellular Localisation | Lymphocyte Activation Gene 3 Protein: Cell MembraneSingle-Pass Type I Membrane ProteinSecreted Lymphocyte Activation Gene 3 Protein: SecretedProduced Following Cleavage Of The Main Chain |
| Alternative Nanobody Names | Anti-Lymphocyte Activation Gene 3 Protein nanobodyAnti-Lag-3 nanobodyAnti-Activation-Induced Cytidine Deaminase-Linked Autoimmunity Protein nanobodyAnti-Aida nanobodyAnti-Cd Antigen Cd223 Cleaved Into - Secreted Lymphocyte Activation Gene 3 Protein nanobodyAnti-Slag-3 nanobodyAnti-Lag3 nanobody |
Information sourced from Uniprot.org