• Immunofluorescence analysis of HeLa cells using CD177 antibody (STJ115558). Blue: DAPI for nuclear staining.

Anti-CD177 antibody (35-285) (STJ115558)

SKU:
STJ115558

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Host: Rabbit
Applications: IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD177 (35-285) is suitable for use in Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD177
Gene ID: 57126
Uniprot ID: CD177_HUMAN
Immunogen Region: 35-285
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human CD177 (NP_065139.2).
Immunogen Sequence: VSDLPRQWTPKNTSCDSGLG CQDTLMLIESGPQVSLVLSK GCTEAKDQEPRVTEHRMGPG LSLISYTFVCRQEDFCNNLV NSLPLWAPQPPADPGSLRCP VCLSMEGCLEGTTEEICPKG TTHCYDGLLRLRGGGIFSNL RVQGCMPQPVCNLLNGTQEI GPVGMTENCDMKDFLTCHRG TTIMTHGNLAQEPTDWTTSN TEMCEVGQVCQETLLLLDVG LTSTLVGTKGCSTVGAQNS
Tissue Specificity Highly expressed in normal bone marrow and weakly expressed in fetal liver. During neutrophil differentiation, expression begins at the metamyelocyte stage and continues throughout the subsequent stages (at protein level). Expressed by a subset of mature neutrophils (at protein level). The percentage of neutrophils expressing CD177 varies across the population. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).
Post Translational Modifications N-glycosylated. A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).
Function In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production. In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration. Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro. However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo. Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils. By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis.
Protein Name Cd177 Antigen
Human Neutrophil Alloantigen 2a
Hna-2a
Nb1 Glycoprotein
Nb1 Gp
Polycythemia Rubra Vera Protein 1
Prv-1
Cd Antigen Cd177
Database Links Reactome: R-HSA-140875
Reactome: R-HSA-202733
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Membrane Raft
Gpi-Like-Anchor
Secreted
Cytoplasmic Granule Membrane
Cell Projection
Lamellipodium
Cell Surface Expression On Neutrophils Is Increased Upon Tnf-Alpha
Fmlp Or Cxcl8/Il8-Mediated Stimulation
In Neutrophils
Stored Predominantly In Secondary And Tertiary Granules
Can Also Be Shedded From The Cell Membrane
Localizes To Lamellar Protrusions In Spreading Neutrophils
Alternative Antibody Names Anti-Cd177 Antigen antibody
Anti-Human Neutrophil Alloantigen 2a antibody
Anti-Hna-2a antibody
Anti-Nb1 Glycoprotein antibody
Anti-Nb1 Gp antibody
Anti-Polycythemia Rubra Vera Protein 1 antibody
Anti-Prv-1 antibody
Anti-Cd Antigen Cd177 antibody
Anti-CD177 antibody
Anti-NB1 antibody
Anti-PRV1 antibody
Anti-UNQ595 antibody
Anti-PRO1181 antibody

Information sourced from Uniprot.org

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