• Western blot analysis of lysates from K562 cells, using Cyclin E1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of A549 cells, using Cyclin E1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Cyclin E1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of MCF7 cells using Cyclin E1 Polyclonal Antibody diluted at 1：500
  • Western blot analysis of various cells using Cyclin E1 Polyclonal Antibody diluted at 1：500
  • Immunofluorescence analysis of rat-spleen tissue. 1, Cyclin E1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Hela cell. 1, Cyclin E1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CCNE1 antibody (91-140 aa) (STJ92541)

SKU:
STJ92541

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-G1/S-specific cyclin-E1 (91-140 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNE1
Gene ID: 898
Uniprot ID: CCNE1_HUMAN
Immunogen Region: 91-140 aa
Specificity: Cyclin E1 Polyclonal Antibody detects endogenous levels of Cyclin E1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Cyclin E1 at the amino acid range 91-140
Post Translational Modifications Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2.appears to occur independently of phosphorylation.
Function Essential for the control of the cell cycle at the G1/S (start) transition.
Protein Name G1/S-Specific Cyclin-E1
Database Links Reactome: R-HSA-1538133
Reactome: R-HSA-187577
Reactome: R-HSA-2559586
Reactome: R-HSA-390471
Reactome: R-HSA-6804116
Reactome: R-HSA-69017
Reactome: R-HSA-69200
Reactome: R-HSA-69202
Reactome: R-HSA-69205
Reactome: R-HSA-69231
Reactome: R-HSA-69563
Reactome: R-HSA-8849470
Reactome: R-HSA-9661069
Reactome: R-HSA-9706019
Cellular Localisation Nucleus
Alternative Antibody Names Anti-G1/S-Specific Cyclin-E1 antibody
Anti-CCNE1 antibody
Anti-CCNE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance