• Western blot analysis of lysates from wild type (WT) and Catalasealase knockout (KO) HeLa cells, using [KO Validated] Catalase Rabbit polyclonal antibody (STJ119991) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CAT antibody (1-225) (STJ119991)

SKU:
STJ119991

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAT (1-225) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAT
Gene ID: 847
Uniprot ID: CATA_HUMAN
Immunogen Region: 1-225
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human Catalasealase (NP_001743.1).
Immunogen Sequence: MADSRDPASDQMQHWKEQRA AQKADVLTTGAGNPVGDKLN VITVGPRGPLLVQDVVFTDE MAHFDRERIPERVVHAKGAG AFGYFEVTHDITKYSKAKVF EHIGKKTPIAVRFSTVAGES GSADTVRDPRGFAVKFYTED GNWDLVGNNTPIFFIRDPIL FPSFIHSQKRNPQTHLKDPD MVWDFWSLRPESLHQVSFLF SDRGIPDGHRHMNGYGSHTF KLVNA
Function Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Protein Name Catalase
Database Links Reactome: R-HSA-3299685
Reactome: R-HSA-6798695
Reactome: R-HSA-9033241
Reactome: R-HSA-9615017
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-Catalase antibody
Anti-CAT antibody

Information sourced from Uniprot.org

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