| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human, Mouse, Rat |
| Note: |
FOR RESEARCH USE ONLY (RUO). |
| Short Description: |
Rabbit monoclonal antibody anti-active + pro Caspase-3 is suitable for use in Western Blot. |
| Clonality: |
Monoclonal |
| Clone ID: |
ARC0133 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS containing 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store in a freezer at-20°C and avoid freeze-thaw cycles. |
| Gene Symbol: |
CASP3 |
| Gene ID: |
836 |
| Uniprot ID: |
CASP3_HUMAN |
| Immunogen: |
A synthesized peptide derived from human active + pro Caspase-3. |
| Tissue Specificity | Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. |
| Post Translational Modifications | Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. |
| Protein Name | Caspase-3Casp-3ApopainCysteine Protease Cpp32Cpp-32Protein YamaSrebp Cleavage Activity 1Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12 |
| Database Links | Reactome: R-HSA-111459Reactome: R-HSA-111463Reactome: R-HSA-111464Reactome: R-HSA-111465Reactome: R-HSA-111469Reactome: R-HSA-140342Reactome: R-HSA-1474228Reactome: R-HSA-2028269Reactome: R-HSA-205025Reactome: R-HSA-211736Reactome: R-HSA-264870Reactome: R-HSA-351906Reactome: R-HSA-418889Reactome: R-HSA-449836Reactome: R-HSA-5620971 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Caspase-3 antibodyAnti-Casp-3 antibodyAnti-Apopain antibodyAnti-Cysteine Protease Cpp32 antibodyAnti-Cpp-32 antibodyAnti-Protein Yama antibodyAnti-Srebp Cleavage Activity 1 antibodyAnti-Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12 antibodyAnti-CASP3 antibodyAnti-CPP32 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
