• Immunohistochemistry of paraffin-embedded human gastric cancer using C-FOS antibody (STJ23686) at dilution of 1:150 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse brain using C-FOS antibody (STJ23686) at dilution of 1:150 (40x lens).
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using FOS antibody (STJ23686) at dilution of 1:100 (40x lens).

Anti-c-Fos antibody (STJ23686)

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Host: Rabbit
Applications: IHC
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal primary antibody against fos.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: IHC: 1:50-1:200
Gene ID: 2353
Gene Symbol: FOS
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 90-190 of human c-Fos (NP_005243.1).
Background The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Post Translational Modifications Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation. Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232. In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis.
Database Links Reactome: R-HSA-2559580
Cellular Localisation Nucleus
Endoplasmic Reticulum
In Quiescent Cells
Present In Very Small Amounts In The Cytosol
Following Induction Of Cell Growth
First Localizes To The Endoplasmic Reticulum And Only Later To The Nucleus
Localization At The Endoplasmic Reticulum Requires Dephosphorylation At Tyr-10 And Tyr-30
Protein Name Proto-Oncogene C-Fos
Cellular Oncogene Fos
G0/G1 Switch Regulatory Protein 7
Alternative Names Anti-Proto-Oncogene C-Fos antibody
Anti-Cellular Oncogene Fos antibody
Anti-G0/G1 Switch Regulatory Protein 7 antibody
Anti-FOS antibody
Anti-G0S7 antibody

Information sourced from uniprot.org

6 months standard