• WB on marmoset brain lysate prepared in Ripa buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:2000 incubated at 4C overnight.
  • IHC on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Anti-c Fos antibody (STJ13100241)

SKU:
STJ13100241
Shipping:
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Current Stock:
Host: NZ White Rabbit
Applications: IHC, WB
Reactivity: Rat, Mouse, Marmoset
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: NZ White Rabbit polyclonal primary antibody against c Fos.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Shipped as lyophilised. Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Purification: Whole serum
Storage Instruction: Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Dilution Range: Use at a dilution of 1:1000 to 1:2000. The optimal dilution should be determined by the end user. Not yet tested in other applications. For WB, the lysate must be prepared in G1 lysing buffer which is:Ripa lysing buffer containing 1% Triton X-100; 1%
Uniprot ID: FOS_HUMAN
Gene ID: 2353
Gene Symbol: FOS
Immunogen: A synthetic peptide from human cFos protein conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in many other species including rat and mouse.
6 months standard