• Western blot analysis of extracts of various cell lines, using TUBB antibody (STJ11103688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of various lysates, using Beta-Tubulin rabbit polyclonal antibody (STJ11103688) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded HepG2 cells using Beta-Tubulin antibody (STJ11103688) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded HepG2 cells treated by oleic acid using Beta-Tubulin antibody (STJ11103688) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of A431 cells using Beta-Tubulin antibody (STJ11103688) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Beta-Tubulin antibody (STJ11103688) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Anti-Beta-Tubulin antibody (40-260) (STJ11103688)

Anti-Beta-Tubulin antibody (40-260) (STJ11103688)

SKU:
STJ11103688

£140.50 - £212.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Beta-Tubulin (40-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TUBB
Gene ID: 203068
Uniprot ID: TBB5_HUMAN
Immunogen Region: 40-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human Beta-Tubulin (NP_006077.2).
Immunogen Sequence: SDLQLERISVYYNEASSHKY VPRAILVDLEPGTMDSVRSG AFGHLFRPDNFIFGQSGAGN NWAKGHYTEGAELVDSVLDV VRKECENCDCLQGFQLTHSL GGGTGSGMGTLLISKVREEY PDRIMNTFSVVPSPKVSDTV VEPYNATLSIHQLVENTDET YCIDNEALYDICFRTLKLAT PTYGDLNHLVSATMSGVTTS LRFPGQLNADLRKLAVNMVP F
Tissue Specificity Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Post Translational Modifications Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility (Probable). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Protein Name Tubulin Beta Chain
Tubulin Beta-5 Chain
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-6798695
Reactome: R-HSA-8854518
Reactome: R-HSA-9679191
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Tubulin Beta Chain antibody
Anti-Tubulin Beta-5 Chain antibody
Anti-TUBB antibody
Anti-TUBB5 antibody
Anti-OK antibody
Anti-SW-cl.56 antibody

Information sourced from Uniprot.org

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