| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Beta-TRCP (1-120) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BTRC |
| Gene ID: |
8945 |
| Uniprot ID: |
FBW1A_HUMAN |
| Immunogen Region: |
1-120 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Beta-TrCP/BTRC (NP_378663.1). |
| Immunogen Sequence: |
MDPAEAVLQEKALKFMCSMP RSLWLGCSSLADSMPSLRCL YNPGTGALTAFQNSSEREDC NNGEPPRKIIPEKNSLRQTY NSCARLCLNQETVCLASTAM KTENCVAKTKLANGTSSMIV |
| Tissue Specificity | Expressed in epididymis (at protein level). |
| Post Translational Modifications | Ubiquitinated. Deubiquitinated by OTUD5, promoting its stability. |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE.the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. The SCF(FBXW11) complex also regulates NF-kappa-B by mediating ubiquitination of phosphorylated NFKB1: specifically ubiquitinates the p105 form of NFKB1, leading to its degradation. SCF(BTRC) mediates the ubiquitination of CEP68.this is required for centriole separation during mitosis. SCF(BTRC) mediates the ubiquitination and subsequent degradation of nuclear NFE2L1. Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3. Mediates ubiquitination of REST, thereby leading to its proteasomal degradation. SCF(BTRC) mediates the ubiquitination and subsequent proteasomal degradation of KLF4.thereby negatively regulating cell pluripotency maintenance and embryogenesis. SCF(BTRC) acts as a regulator of mTORC1 signaling pathway by catalyzing ubiquitination and subsequent proteasomal degradation of phosphorylated DEPTOR, TFE3 and MITF. |
| Protein Name | F-Box/Wd Repeat-Containing Protein 1aE3rsikappabEpididymis Tissue Protein Li 2aF-Box And Wd Repeats Protein Beta-TrcpPikappabalpha-E3 Receptor Subunit |
| Database Links | Reactome: R-HSA-1169091Reactome: R-HSA-1170546Reactome: R-HSA-174113Reactome: R-HSA-180534Reactome: R-HSA-195253Reactome: R-HSA-202424Reactome: R-HSA-2565942Reactome: R-HSA-2871837Reactome: R-HSA-3769402Reactome: R-HSA-400253Reactome: R-HSA-5607761Reactome: R-HSA-5607764Reactome: R-HSA-5610780Reactome: R-HSA-5610783Reactome: R-HSA-5610785Reactome: R-HSA-5676590Reactome: R-HSA-5684264Reactome: R-HSA-8951664Reactome: R-HSA-9020702Reactome: R-HSA-9762114Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-F-Box/Wd Repeat-Containing Protein 1a antibodyAnti-E3rsikappab antibodyAnti-Epididymis Tissue Protein Li 2a antibodyAnti-F-Box And Wd Repeats Protein Beta-Trcp antibodyAnti-Pikappabalpha-E3 Receptor Subunit antibodyAnti-BTRC antibodyAnti-BTRCP antibodyAnti-FBW1A antibodyAnti-FBXW1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
