• Western blot analysis of chicken cell lysis using Antibody diluted at 1:1000
  • Immunohistochemical analysis of paraffin-embedded Rat Testis Tissue using Bax Mouse mAb diluted at 1:200.
  • Western blot analysis of 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate using Bax Mouse mAb diluted at 1:1000.
  • Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using Bax Mouse mAb diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Bax Mouse mAb diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, Bax Mouse monoclonal antibody (6F11) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, Bax Mouse monoclonal antibody (6F11) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of lysates from 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-BAX antibody (30-110 aa) [6F11] (STJ97725)

SKU:
STJ97725

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: IF/WB/IHC
Reactivity: Human/Rat/Mouse/Chicken/Silkworm
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Apoptosis regulator BAX (30-110 aa) is suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 6F11
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:1000-2000
IHC 1:100-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAX
Gene ID: 581
Uniprot ID: BAX_HUMAN
Immunogen Region: 30-110 aa
Specificity: Bax protein detects endogenous levels of BAX
Immunogen: Synthetic peptide of Bax at the amino acid range of 30-110
Post Translational Modifications Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12.
Function Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Protein Name Apoptosis Regulator Bax
Bcl-2-Like Protein 4
Bcl2-L-4
Database Links Reactome: R-HSA-111457
Reactome: R-HSA-114294
Reactome: R-HSA-5620971
Reactome: R-HSA-6803204
Reactome: R-HSA-6804114
Reactome: R-HSA-8878166
Reactome: R-HSA-9603505
Cellular Localisation Isoform Alpha: Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Cytoplasm
Nucleus
Colocalizes With 14-3-3 Proteins In The Cytoplasm
Under Stress Conditions
Undergoes A Conformation Change That Causes Release From Jnk-Phosphorylated 14-3-3 Proteins And Translocation To The Mitochondrion Membrane
Upon Sendai Virus Infection
Recruited To The Mitochondrion Through Interaction With Irf3
Isoform Beta: Cytoplasm
Isoform Gamma: Cytoplasm
Isoform Delta: Cytoplasm
Alternative Antibody Names Anti-Apoptosis Regulator Bax antibody
Anti-Bcl-2-Like Protein 4 antibody
Anti-Bcl2-L-4 antibody
Anti-BAX antibody
Anti-BCL2L4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance