• Human malignant mesothelioma stained with anti-BAP1 antibody using peroxidase-conjugate and AEC chromogen. Note loss of nuclear stain in tumor cells whereas the background cells positive.

Anti-BAP1 antibody [C4] (STJ180014)

SKU:
STJ180014

Current Stock:
Host: Mouse
Applications: IHC-p
Reactivity: Human
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Mouse monoclonal antibody Anti-BAP1 is suitable for use in Immunohistochemistry.
Clonality: Monoclonal
Clone ID: C4
Conjugation: Unconjugated
Isotype: IgG1 Kappa
Formulation: Purified antibody diluted in Tris‐HCI buffer containing stabilizing protein and <0.1% sodium azide.
Dilution Range: 1:100
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: RNF2
Gene ID: 6045
Uniprot ID: RING2_HUMAN
Specificity: Positive control tissue: Malignant Mesothelioma.
Immunogen: Synthetic peptide against 430‐729 of human BAP1
Post Translational Modifications Polyubiquitinated in the presence of UBE2D3 (in vitro). Monoubiquitinated, by auto-ubiquitination.
Function E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes.
Protein Name E3 Ubiquitin-Protein Ligase Ring2
Huntingtin-Interacting Protein 2-Interacting Protein 3
Hip2-Interacting Protein 3
Protein Ding
Ring Finger Protein 1b
Ring1b
Ring Finger Protein 2
Ring Finger Protein Bap-1
Ring-Type E3 Ubiquitin Transferase Ring2
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-3108214
Reactome: R-HSA-3899300
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4655427
Reactome: R-HSA-8939243
Reactome: R-HSA-8943724
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Chromosome
Enriched On Inactive X Chromosome (Xi) In Female Trophoblast Stem (Ts) Cells As Well As Differentiating Embryonic Stem (Es) Cells
The Enrichment On Xi Is Transient During Ts And Es Cell Differentiation
The Association With Xi Is Mitotically Stable In Non-Differentiated Ts Cells
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Ring2 antibody
Anti-Huntingtin-Interacting Protein 2-Interacting Protein 3 antibody
Anti-Hip2-Interacting Protein 3 antibody
Anti-Protein Ding antibody
Anti-Ring Finger Protein 1b antibody
Anti-Ring1b antibody
Anti-Ring Finger Protein 2 antibody
Anti-Ring Finger Protein Bap-1 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Ring2 antibody
Anti-RNF2 antibody
Anti-BAP1 antibody
Anti-DING antibody
Anti-HIPI3 antibody
Anti-RING1B antibody

Information sourced from Uniprot.org

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