| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Potassium-transporting ATPase alpha chain 1/K+ ATPase subunit alpha (800-880 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATP4A |
| Gene ID: |
495 |
| Uniprot ID: |
ATP4A_HUMAN |
| Immunogen Region: |
800-880 aa |
| Specificity: |
ATP4A Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: |
Synthesized peptide derived from the human protein at the amino acid range 800-880 |
| Function | The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell. Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state. |
| Protein Name | Potassium-Transporting Atpase Alpha Chain 1Gastric H(+/K(+ Atpase Subunit AlphaProton Pump |
| Database Links | Reactome: R-HSA-936837 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinLocalized In The Apical Canalicular Membrane Of Parietal Cells |
| Alternative Antibody Names | Anti-Potassium-Transporting Atpase Alpha Chain 1 antibodyAnti-Gastric H(+/K(+ Atpase Subunit Alpha antibodyAnti-Proton Pump antibodyAnti-ATP4A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
