||WB, IHC-P, IF, FC, ELISA
||Mouse monoclonal anti-Ataxin-1 antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA.
||FOR RESEARCH USE ONLY (RUO).
||Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.
||Store at-20°C, and avoid repeat freeze-thaw cycles.
||WB 1:500-1:2000IHC 1:200-1:1000IF 1:200-1:1000FC 1:200-1:400ELISA 1:10000
||Ataxin-1 monoclonal antibody detects endogenous levels of Ataxin-1 protein.
||Purified recombinant fragment of human Ataxin-1 expressed in E. Coli.
| Post Translational Modifications || Ubiquitinated by UBE3A, leading to its degradation by the proteasome. The presence of expanded poly-Gln repeats in spinocerebellar ataxia 1 (SCA1) patients impairs ubiquitination and degradation, leading to accumulation of ATXN1 in neurons and subsequent toxicity. Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract. Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract. |
| Function || Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. In concert with CIC and ATXN1L, involved in brain development. |
| Protein Name || Ataxin-1Spinocerebellar Ataxia Type 1 Protein |
| Cellular Localisation || CytoplasmNucleusColocalizes With Usp7 In The Nucleus |
| Alternative Antibody Names || Anti-Ataxin-1 antibodyAnti-Spinocerebellar Ataxia Type 1 Protein antibodyAnti-ATXN1 antibodyAnti-ATX1 antibodyAnti-SCA1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance