• Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R8 antibody (STJ24013) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R8 antibody (STJ24013).
  • Immunohistochemistry analysis of paraffin-embedded Human colon using Asymmetric DiMethyl-Histone H3-R8 rabbit polyclonal antibody (STJ24013) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat testis using Asymmetric DiMethyl-Histone H3-R8 rabbit polyclonal antibody (STJ24013) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using Asymmetric DiMethyl-Histone H3-R8 rabbit polyclonal antibody (STJ24013) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R8 antibody (STJ24013). Blue: DAPI for nuclear staining.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Asymmetric DiMethyl-Histone H3-R8 antibody (STJ24013) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Di-Methyl-Histone H3-R8 antibody (Around R8) (STJ24013)

SKU:
STJ24013

$286.88 - $677.03
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Di-Methyl-Asymmetric Histone H3-R8 (Around R8) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
ChIP 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: Around R8
Immunogen: A synthetic asymmetric dimethylated peptide around R8 of human histone H3 (NP_003520.1).
Immunogen Sequence: TARKS
Background This gene encodes the Landsteiner-Wiener (LW) blood group antigen (s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. This ICAM protein contains 2 Ig-like C2-type domains and binds to the leukocyte adhesion LFA-1 protein. The molecular basis of the LW (A) /LW (B) blood group antigens is a single aa variation at position 100; Gln-100=LW (A) and Arg-100=LW (B). Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Information sourced from Uniprot.org

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