| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ASPN (100-379) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ASPN |
| Gene ID: |
54829 |
| Uniprot ID: |
ASPN_HUMAN |
| Immunogen Region: |
100-379 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 100-379 of human ASPN (NP_060150.4). |
| Immunogen Sequence: |
PFDTRMLDLQNNKIKEIKEN DFKGLTSLYGLILNNNKLTK IHPKAFLTTKKLRRLYLSHN QLSEIPLNLPKSLAELRIHE NKVKKIQKDTFKGMNALHVL EMSANPLDNNGIEPGAFEGV TVFHIRIAEAKLTSVPKGLP PTLLELHLDYNKISTVELED FKRYKELQRLGLGNNKITDI ENGSLANIPRVREIHLENNK LKKIPSGLPELKYLQIIFLH SNSIARVGVNDFCPTVPKM |
| Tissue Specificity | Higher levels in osteoarthritic articular cartilage, aorta, uterus. Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands. Low expression in trachea, bone marrow, and lung. Colocalizes with TGFB1 in chondrocytes within osteoarthritic (OA) lesions of articular cartilage. |
| Post Translational Modifications | There is no serine/glycine dipeptide sequence expected for the attachment of O-linked glycosaminoglycans and this is probably not a proteoglycan. The O-linked polysaccharide on 54-Ser is probably the mucin type linked to GalNAc. The N-linked glycan at Asn-282 is composed of variable structures of GlcNAc, mannose, fucose, HexNAc and hexose. |
| Function | Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins. Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity. |
| Protein Name | AsporinPeriodontal Ligament-Associated Protein 1Plap-1 |
| Database Links | Reactome: R-HSA-3000178 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Asporin antibodyAnti-Periodontal Ligament-Associated Protein 1 antibodyAnti-Plap-1 antibodyAnti-ASPN antibodyAnti-PLAP1 antibodyAnti-SLRR1C antibodyAnti-UNQ215 antibodyAnti-PRO241 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
