• Western blot analysis of extracts of various cell lines, using ARHGEF3 antibody (STJ110788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using ARHGEF3 antibody (STJ110788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ARHGEF3 antibody (287-526) (STJ110788)

SKU:
STJ110788

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARHGEF3 (287-526) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGEF3
Gene ID: 50650
Uniprot ID: ARHG3_HUMAN
Immunogen Region: 287-526
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 287-526 of human ARHGEF3 (NP_062455.1).
Immunogen Sequence: LEEAINIIQGIVAEINTKTG ESECRYYKERLLYLEEGQKD SLIDSSRVLCCHGELKNNRG VKLHVFLFQEVLVITRAVTH NEQLCYQLYRQPIPVKDLLL EDLQDGEVRLGGSLRGAFSN NERIKNFFRVSFKNGSQSQT HSLQANDTFNKQQWLNCIRQ AKETVLCAAGQAGVLDSEGS FLNPTTGSRELQGETKLEQM DQSDSESDCSMDTSEVSLDC ERMEQTDSSCGNSRHGESN
Tissue Specificity Widely expressed. Highest levels are found in adult brain and skeletal muscle. Lower levels are found in heart and kidney.
Function Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.
Protein Name Rho Guanine Nucleotide Exchange Factor 3
Exchange Factor Found In Platelets And Leukemic And Neuronal Tissues
Xpln
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-416482
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Rho Guanine Nucleotide Exchange Factor 3 antibody
Anti-Exchange Factor Found In Platelets And Leukemic And Neuronal Tissues antibody
Anti-Xpln antibody
Anti-ARHGEF3 antibody

Information sourced from Uniprot.org

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