• Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and ARHGEF1 knockout (KO) 293T cells, using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARHGEF1 antibody (783-912) (STJ116239)

SKU:
STJ116239

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARHGEF1 (783-912) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGEF1
Gene ID: 9138
Uniprot ID: ARHG1_HUMAN
Immunogen Region: 783-912
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2).
Immunogen Sequence: PLLSSSENGNGGRETSPADA RTERILSDLLPFCRPGPEGQ LAATALRKVLSLKQLLFPAE EDNGAGPPRDGDGVPGGGPL SPARTQEIQENLLSLEETMK QLEELEEEFCRLRPLLSQLG GNSVPQPGCT
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2.
Function Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.
Protein Name Rho Guanine Nucleotide Exchange Factor 1
115 Kda Guanine Nucleotide Exchange Factor
P115-Rhogef
P115rhogef
Sub1.5
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-416482
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Cellular Localisation Cytoplasm
Membrane
Translocated To The Membrane By Activated Gna13 Or Lpa Stimulation
Alternative Antibody Names Anti-Rho Guanine Nucleotide Exchange Factor 1 antibody
Anti-115 Kda Guanine Nucleotide Exchange Factor antibody
Anti-P115-Rhogef antibody
Anti-P115rhogef antibody
Anti-Sub1.5 antibody
Anti-ARHGEF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance