• Western blot analysis of lysates from wild type (WT) and ARF1 knockout (KO) HeLa cells, using [KO Validated] ARF1 Rabbit polyclonal antibody (STJ11100853) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARF1 antibody (1-181) (STJ11100853)

SKU:
STJ11100853

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARF1 (1-181) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARF1
Gene ID: 375
Uniprot ID: ARF1_HUMAN
Immunogen Region: 1-181
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ARF1 (NP_001649.1).
Immunogen Sequence: MGNIFANLFKGLFGKKEMRI LMVGLDAAGKTTILYKLKLG EIVTTIPTIGFNVETVEYKN ISFTVWDVGGQDKIRPLWRH YFQNTQGLIFVVDSNDRERV NEAREELMRMLAEDELRDAV LLVFANKQDLPNAMNAAEIT DKLGLHSLRHRNWYIQATCA TSGDGLYEGLDWLSNQLRNQ K
Post Translational Modifications (Microbial infection) Demyristoylated by S.flexneri cysteine protease IpaJ which cleaves the peptide bond between N-myristoylated Gly-2 and Asn-3.
Function Small GTPase involved in protein trafficking between different compartments. Modulates vesicle budding and uncoating within the Golgi complex. In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity.the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD). (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Protein Name Adp-Ribosylation Factor 1
Database Links Reactome: R-HSA-1660499
Reactome: R-HSA-1660514
Reactome: R-HSA-167590
Reactome: R-HSA-199992
Reactome: R-HSA-2132295
Reactome: R-HSA-432720
Reactome: R-HSA-432722
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-6811438
Reactome: R-HSA-8950505
Cellular Localisation Golgi Apparatus Membrane
Lipid-Anchor
Cytoplasmic Side
Synapse
Synaptosome
Postsynaptic Density
In The Gdp-Bound Form
Associates Transiently With The Membranes Via Its Myristoylated N-Terminus Where Guanine Nucleotide-Exchange Factor (Gef)-Mediated Nucleotide Exchange Occurs
Following Nucleotide Exchange
The Gtp-Bound Form Undergoes A Conformational Change
Leading To The Exposure Of A Myristoylated N-Terminal Amphipathic Helix That Provides Stable Membrane Anchorage
Alternative Antibody Names Anti-Adp-Ribosylation Factor 1 antibody
Anti-ARF1 antibody

Information sourced from Uniprot.org

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