• Western blot analysis of extracts of various cell lines, using Annexin A2 rabbit monoclonal antibody (STJ11101300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of Annexin A2 in paraffin-embedded human cervix cancer tissue using Annexin A2 rabbit monoclonal antibody (STJ11101300) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Annexin A2 in paraffin-embedded human colon carcinoma tissue using Annexin A2 rabbit monoclonal antibody (STJ11101300) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Annexin A2 in paraffin-embedded mouse colon tissue using Annexin A2 rabbit monoclonal antibody (STJ11101300) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Annexin A2 in paraffin-embedded mouse liver tissue using Annexin A2 rabbit monoclonal antibody (STJ11101300) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Annexin A2 in paraffin-embedded mouse spleen tissue using Annexin A2 rabbit monoclonal antibody (STJ11101300) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Annexin A2 in paraffin-embedded rat colon tissue using Annexin A2 rabbit monoclonal antibody (STJ11101300) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-ANXA2 antibody (1-100) [S0MR] (STJ11101300)

SKU:
STJ11101300

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Annexin A2 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANXA2
Gene ID: 302
Uniprot ID: ANXA2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Annexin A2 (P07355).
Immunogen Sequence: MSTVHEILCKLSLEGDHSTP PSAYGSVKAYTNFDAERDAL NIETAIKTKGVDEVTIVNIL TNRSNAQRQDIAFAYQRRTK KELASALKSALSGHLETVIL
Post Translational Modifications Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated.
Function Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.
Protein Name Annexin A2
Annexin Ii
Annexin-2
Calpactin I Heavy Chain
Calpactin-1 Heavy Chain
Chromobindin-8
Lipocortin Ii
Placental Anticoagulant Protein Iv
Pap-Iv
Protein I
P36
Database Links Reactome: R-HSA-445355
Reactome: R-HSA-6798695
Reactome: R-HSA-75205
Reactome: R-HSA-8950505
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Melanosome
In The Lamina Beneath The Plasma Membrane
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Translocated From The Cytoplasm To The Cell Surface Through A Golgi-Independent Mechanism
Alternative Antibody Names Anti-Annexin A2 antibody
Anti-Annexin Ii antibody
Anti-Annexin-2 antibody
Anti-Calpactin I Heavy Chain antibody
Anti-Calpactin-1 Heavy Chain antibody
Anti-Chromobindin-8 antibody
Anti-Lipocortin Ii antibody
Anti-Placental Anticoagulant Protein Iv antibody
Anti-Pap-Iv antibody
Anti-Protein I antibody
Anti-P36 antibody
Anti-ANXA2 antibody
Anti-ANX2 antibody
Anti-ANX2L4 antibody
Anti-CAL1H antibody
Anti-LPC2D antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance