• Western blot analysis of extracts of Mouse liver, using Angiotensinogen Rabbit mAb (STJ11101390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using Angiotensinogen Rabbit mAb (STJ11101390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 3s.

Anti-Angiotensinogen-antibody [ARC0677] (STJ11101390)

SKU:
STJ11101390

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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody anti-Angiotensinogen is suitable for use in Western Blot.
Clonality: Monoclonal
Clone ID: ARC0677
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: AGT
Gene ID: 183
Uniprot ID: ANGT_HUMAN
Immunogen: A synthesized peptide derived from human Angiotensinogen
Tissue Specificity Expressed by the liver and secreted in plasma.
Post Translational Modifications Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor. In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin.
Function Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Angiotensin-3: stimulates aldosterone release. Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
Protein Name Angiotensinogen
Serpin A8 Cleaved Into - Angiotensin-1
Angiotensin 1-10
Angiotensin I
Ang I - Angiotensin-2
Angiotensin 1-8
Angiotensin Ii
Ang Ii - Angiotensin-3
Angiotensin 2-8
Angiotensin Iii
Ang Iii
Des-Asp1-Angiotensin Ii - Angiotensin-4
Angiotensin 3-8
Angiotensin Iv
Ang Iv - Angiotensin 1-9 - Angiotensin 1-7 - Angiotensin 1-5 - Angiotensin 1-4
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2022377
Reactome: R-HSA-375276
Reactome: R-HSA-416476
Reactome: R-HSA-418594
Cellular Localisation Secreted
Alternative Antibody Names Anti-Angiotensinogen antibody
Anti-Serpin A8 Cleaved Into - Angiotensin-1 antibody
Anti-Angiotensin 1-10 antibody
Anti-Angiotensin I antibody
Anti-Ang I - Angiotensin-2 antibody
Anti-Angiotensin 1-8 antibody
Anti-Angiotensin Ii antibody
Anti-Ang Ii - Angiotensin-3 antibody
Anti-Angiotensin 2-8 antibody
Anti-Angiotensin Iii antibody
Anti-Ang Iii antibody
Anti-Des-Asp1-Angiotensin Ii - Angiotensin-4 antibody
Anti-Angiotensin 3-8 antibody
Anti-Angiotensin Iv antibody
Anti-Ang Iv - Angiotensin 1-9 - Angiotensin 1-7 - Angiotensin 1-5 - Angiotensin 1-4 antibody
Anti-AGT antibody
Anti-SERPINA8 antibody

Information sourced from Uniprot.org

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