• Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells, using 3 Mu g ANAPC5 antibody (STJ29189). Western blot was performed from the immunoprecipitate using ANAPC5 antibody (STJ29189) at a dilution of 1:1000.
  • Immunohistochemistry analysis of ANAPC5 in paraffin-embedded human kidney using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ANAPC5 in paraffin-embedded rat kidney using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and ANAPC5 knockout (KO) 293T cells, using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ANAPC5 antibody (1-250) (STJ29189)

SKU:
STJ29189

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANAPC5 (1-250) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANAPC5
Gene ID: 51433
Uniprot ID: APC5_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2).
Immunogen Sequence: MASVHESLYFNPMMTNGVVH ANVFGIKDWVTPYKIAVLVL LNEMSRTGEGAVSLMERRRL NQLLLPLLQGPDITLSKLYK LIEESCPQLANSVQIRIKLM AEGELKDMEQFFDDLSDSFS GTEPEVHKTSVVGLFLRHMI LAYSKLSFSQVFKLYTALQQ YFQNGEKKTVEDADMELTSR DEGERKMEKEELDVSVREEE VSCSGPLSQKQAEFFLSQQA SLLKNDETKALTPASLQKE
Function Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Protein Name Anaphase-Promoting Complex Subunit 5
Apc5
Cyclosome Subunit 5
Database Links Reactome: R-HSA-141430
Reactome: R-HSA-174048
Reactome: R-HSA-174084
Reactome: R-HSA-174154
Reactome: R-HSA-174178
Reactome: R-HSA-174184
Reactome: R-HSA-176407
Reactome: R-HSA-176408
Reactome: R-HSA-176409
Reactome: R-HSA-176412
Reactome: R-HSA-179409
Reactome: R-HSA-2467813
Reactome: R-HSA-2559582
Reactome: R-HSA-68867
Reactome: R-HSA-69017
Reactome: R-HSA-8853884
Reactome: R-HSA-9687136
Reactome: R-HSA-983168
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Spindle
Alternative Antibody Names Anti-Anaphase-Promoting Complex Subunit 5 antibody
Anti-Apc5 antibody
Anti-Cyclosome Subunit 5 antibody
Anti-ANAPC5 antibody
Anti-APC5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance