• Western blot analysis of extracts of various cell lines, using AMPK Beta 1 rabbit monoclonal antibody (STJ11102047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of mouse brain, using AMPK Beta 1 rabbit monoclonal antibody (STJ11102047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using AMPK Beta 1 rabbit monoclonal antibody (STJ11102047) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using AMPK Beta 1 rabbit monoclonal antibody (STJ11102047) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of NIH/3T3 cells using AMPK Beta 1 rabbit monoclonal antibody (STJ11102047, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Confocal imaging of MCF7 cells using AMPK Beta 1 rabbit monoclonal antibody (STJ11102047, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-PRKAB1 antibody (50-150) [S7MR] (STJ11102047)

SKU:
STJ11102047

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-AMPK Beta 1 (50-150) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAB1
Gene ID: 5564
Uniprot ID: AAKB1_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human AMPK Beta 1 (Q9Y478).
Immunogen Sequence: EEIKAPEKEEFLAWQHDLEV NDKAPAQARPTVFRWTGGGK EVYLSGSFNNWSKLPLTRSH NNFVAILDLPEGEHQYKFFV DGQWTHDPSEPIVTSQLGTV N
Post Translational Modifications Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1.leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
Function Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Protein Name 5'-Amp-Activated Protein Kinase Subunit Beta-1
Ampk Subunit Beta-1
Ampkb
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-1632852
Reactome: R-HSA-2151209
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-6804756
Reactome: R-HSA-9613354
Reactome: R-HSA-9619483
Alternative Antibody Names Anti-5'-Amp-Activated Protein Kinase Subunit Beta-1 antibody
Anti-Ampk Subunit Beta-1 antibody
Anti-Ampkb antibody
Anti-PRKAB1 antibody
Anti-AMPK antibody

Information sourced from Uniprot.org

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