• Immunohistochemistry analysis of paraffin-embedded mouse kidney using Alkaline Phosphatase (Alkaline Phosphatase (ALPL) ) Rabbit polyclonal antibody (STJ114272) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of HeLa cells, using Alkaline Phosphatase (ALPL) antibody (STJ114272) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ALPL antibody (230-440) (STJ114272)

SKU:
STJ114272

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALPL (230-440) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALPL
Gene ID: 249
Uniprot ID: PPBT_HUMAN
Immunogen Region: 230-440
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-440 of human Alkaline Phosphatase (ALPL) (NP_000469.3).
Immunogen Sequence: NKTDVEYESDEKARGTRLDG LDLVDTWKSFKPRYKHSHFI WNRTELLTLDPHNVDYLLGL FEPGDMQYELNRNNVTDPSL SEMVVVAIQILRKNPKGFFL LVEGGRIDHGHHEGKAKQAL HEAVEMDRAIGQAGSLTSSE DTLTVVTADHSHVFTFGGYT PRGNSIFGLAPMLSDTDKKP FTAILYGNGPGYKVVGGERE NVSMVDYAHNN
Post Translational Modifications N-glycosylated.
Function Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. Has broad substrate specificity and can hydrolyze a considerable variety of compounds: however, only a few substrates, such as diphosphate (inorganic pyrophosphate.PPi), pyridoxal 5'-phosphate (PLP) and N-phosphocreatine are natural substrates. Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate: it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix. Also promotes dephosphorylation of osteopontin (SSP1), an inhibitor of hydroxyapatite crystallization in its phosphorylated state.it is however unclear whether ALPL/TNAP mediates SSP1 dephosphorylation via a direct or indirect manner. Catalyzes dephosphorylation of PLP to pyridoxal (PL), the transportable form of vitamin B6, in order to provide a sufficient amount of PLP in the brain, an essential cofactor for enzymes catalyzing the synthesis of diverse neurotransmitters. Additionally, also able to mediate ATP degradation in a stepwise manner to adenosine, thereby regulating the availability of ligands for purinergic receptors. Also capable of dephosphorylating microbial products, such as lipopolysaccharides (LPS) as well as other phosphorylated small-molecules, such as poly-inosine:cytosine (poly I:C). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating hydrolysis of N-phosphocreatine to initiate a futile cycle of creatine dephosphorylation and phosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work.
Protein Name Alkaline Phosphatase - Tissue-Nonspecific Isozyme
Ap-Tnap
Tns-Alp
Tnsalp
Alkaline Phosphatase Liver/Bone/Kidney Isozyme
Phosphoamidase
Phosphocreatine Phosphatase
Database Links Reactome: R-HSA-163125
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Vesicle Membrane
Mitochondrion Membrane
Mitochondrion Intermembrane Space
Localizes To Special Class Of Extracellular Vesicles
Named Matrix Vesicles (Mvs)
Which Are Released By Osteogenic Cells
Localizes To The Mitochondria Of Thermogenic Fat Cells: Tethered To Mitochondrial Membranes Via A Gpi-Anchor And Probably Resides In The Mitochondrion Intermembrane Space
Alternative Antibody Names Anti-Alkaline Phosphatase - Tissue-Nonspecific Isozyme antibody
Anti-Ap-Tnap antibody
Anti-Tns-Alp antibody
Anti-Tnsalp antibody
Anti-Alkaline Phosphatase Liver/Bone/Kidney Isozyme antibody
Anti-Phosphoamidase antibody
Anti-Phosphocreatine Phosphatase antibody
Anti-ALPL antibody

Information sourced from Uniprot.org

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