• Confocal imaging of NIH/3T3 cells using AP2A1 Rabbit monoclonal antibody (STJ11102071, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunofluorescence analysis of C6 cells using AP2A1 Rabbit monoclonal antibody (STJ11102071) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using AP2A1 Rabbit monoclonal antibody (STJ11102071) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AP2A1 antibody (1-100) [S1MR] (STJ11102071)

SKU:
STJ11102071

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Alpha1 adaptin (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AP2A1
Gene ID: 160
Uniprot ID: AP2A1_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human AP2A1 (O95782).
Immunogen Sequence: MPAVSKGDGMRGLAVFISDI RNCKSKEAEIKRINKELANI RSKFKGDKALDGYSKKKYVC KLLFIFLLGHDIDFGHMEAV NLLSSNKYTEKQIGYLFISV
Tissue Specificity Expressed in the brain (at protein level). Isoform A: Expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B: Widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.
Function Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-FILMV (Y-X-X-Phi) and ED-X-X-X-L-LI endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the ED-X-X-X-L-LI motif.
Protein Name Ap-2 Complex Subunit Alpha-1
100 Kda Coated Vesicle Protein A
Adaptor Protein Complex Ap-2 Subunit Alpha-1
Adaptor-Related Protein Complex 2 Subunit Alpha-1
Alpha-Adaptin A
Alpha1-Adaptin
Clathrin Assembly Protein Complex 2 Alpha-A Large Chain
Plasma Membrane Adaptor Ha2/Ap2 Adaptin Alpha A Subunit
Database Links Reactome: R-HSA-167590
Reactome: R-HSA-177504
Reactome: R-HSA-182218
Reactome: R-HSA-2132295
Reactome: R-HSA-3928665
Reactome: R-HSA-416993
Reactome: R-HSA-437239
Reactome: R-HSA-5099900
Reactome: R-HSA-5140745
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8866427
Reactome: R-HSA-8964038
Reactome: R-HSA-9679191
Cellular Localisation Cell Membrane
Membrane
Coated Pit
Peripheral Membrane Protein
Cytoplasmic Side
Ap-2 Appears To Be Excluded From Internalizing Ccvs And To Disengage From Sites Of Endocytosis Seconds Before Internalization Of The Nascent Ccv
Alternative Antibody Names Anti-Ap-2 Complex Subunit Alpha-1 antibody
Anti-100 Kda Coated Vesicle Protein A antibody
Anti-Adaptor Protein Complex Ap-2 Subunit Alpha-1 antibody
Anti-Adaptor-Related Protein Complex 2 Subunit Alpha-1 antibody
Anti-Alpha-Adaptin A antibody
Anti-Alpha1-Adaptin antibody
Anti-Clathrin Assembly Protein Complex 2 Alpha-A Large Chain antibody
Anti-Plasma Membrane Adaptor Ha2/Ap2 Adaptin Alpha A Subunit antibody
Anti-AP2A1 antibody
Anti-ADTAA antibody
Anti-CLAPA1 antibody

Information sourced from Uniprot.org

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