| Host: | Rabbit |
| Applications: | WB, IHC, IF |
| Reactivity: | Human, Mouse, Rat |
| Note: | FOR RESEARCH USE ONLY (RUO). |
| Short Description: | Rabbit polyclonal antibody anti-Alpha-Tubulin is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IHC 1:25-1:50IF 1:25-1:100 |
| Storage Instruction: | Store in a freezer at-20°C and avoid freeze-thaw cycles. |
| Gene Symbol: | TUBA4A |
| Gene ID: | 7277 |
| Uniprot ID: | TBA4A_HUMAN |
| Immunogen: | Recombinant protein of human Alpha-Tubulin |
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| Protein Name | Tubulin Alpha-4a ChainAlpha-Tubulin 1Testis-Specific Alpha-TubulinTubulin H2-AlphaTubulin Alpha-1 Chain |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1445148Reactome: R-HSA-190840Reactome: R-HSA-190861Reactome: R-HSA-2132295Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-2565942Reactome: R-HSA-3371497Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-389957Reactome: R-HSA-389960Reactome: R-HSA-389977Reactome: R-HSA-437239Reactome: R-HSA-5610787Reactome: R-HSA-5617833Reactome: R-HSA-5620912Reactome: R-HSA-5620924Reactome: R-HSA-5626467Reactome: R-HSA-5663220Reactome: R-HSA-6807878Reactome: R-HSA-6811434Reactome: R-HSA-6811436Reactome: R-HSA-68877Reactome: R-HSA-8852276Reactome: R-HSA-8854518Reactome: R-HSA-8955332Reactome: R-HSA-9609690Reactome: R-HSA-9609736Reactome: R-HSA-9619483Reactome: R-HSA-9646399Reactome: R-HSA-9648025Reactome: R-HSA-9668328Reactome: R-HSA-983189 |
| Cellular Localisation | CytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Tubulin Alpha-4a Chain antibodyAnti-Alpha-Tubulin 1 antibodyAnti-Testis-Specific Alpha-Tubulin antibodyAnti-Tubulin H2-Alpha antibodyAnti-Tubulin Alpha-1 Chain antibodyAnti-TUBA4A antibodyAnti-TUBA1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
