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Anti-Alpha-Tubulin antibody (1-180) (STJ11103687)

Western blot analysis of extracts of various cell lines, using Alpha-Tubulin rabbit polyclonal antibody (STJ11103687) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit.
Immunofluorescence analysis of U2OS cells using Alpha-Tubulin Rabbit polyclonal antibody (STJ11103687) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat brain using Alpha-Tubulin Antibody (STJ11103687) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using Alpha-Tubulin Rabbit polyclonal antibody (STJ11103687) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat kidney using Alpha-Tubulin Antibody (STJ11103687) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunofluorescence analysis of C6 cells using Alpha-Tubulin Rabbit polyclonal antibody (STJ11103687) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat heart using Alpha-Tubulin Antibody (STJ11103687) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of Alpha-Tubulin in paraffin-embedded rat heart using Alpha-Tubulin Rabbit polyclonal antibody (STJ11103687) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunofluorescence analysis of C6 cells using Alpha-Tubulin rabbit polyclonal antibody (STJ11103687) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of Alpha-Tubulin in paraffin-embedded rat kidney using Alpha-Tubulin Rabbit polyclonal antibody (STJ11103687) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using Alpha-Tubulin rabbit polyclonal antibody (STJ11103687) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of Alpha-Tubulin in paraffin-embedded rat brain using Alpha-Tubulin Rabbit polyclonal antibody (STJ11103687) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunofluorescence analysis of U2OS cells using Alpha-Tubulin rabbit polyclonal antibody (STJ11103687) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using Alpha-Tubulin Rabbit polyclonal antibody (STJ11103687) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-Alpha-Tubulin antibody (1-180) (STJ11103687)

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STJ11103687

£212.50 - £335.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Alpha-Tubulin (1-180) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IHC-P 1:25-1:50 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	TUBA4A
Gene ID:	7277
Uniprot ID:	TBA4A_HUMAN
Immunogen Region:	1-180
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human Alpha-Tubulin (P68366).
Immunogen Sequence:	MRECISVHVGQAGVQMGNAC WELYCLEHGIQPDGQMPSDK TIGGGDDSFTTFFCETGAGK HVPRAVFVDLEPTVIDEIRN GPYRQLFHPEQLITGKEDAA NNYARGHYTIGKEIIDPVLD RIRKLSDQCTGLQGFLVFHS FGGGTGSGFTSLLMERLSVD YGKKSKLEFSIYPAPQVSTA

Post Translational Modifications	Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. Although this tubulin does not encode a C-terminal tyrosine, a C-terminal tyrosine can be added post-translationally by the tubulin tyrosine ligase (TTL). It can then undergo a detyrosination cycle by the tubulin tyrosine carboxypeptidase (KIAA0895L/MATCAP).
Function	Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Protein Name	Tubulin Alpha-4a Chain Alpha-Tubulin 1 Testis-Specific Alpha-Tubulin Tubulin H2-Alpha Tubulin Alpha-1 Chain
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-1445148 Reactome: R-HSA-190840 Reactome: R-HSA-190861 Reactome: R-HSA-2132295 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-2565942 Reactome: R-HSA-3371497 Reactome: R-HSA-380259 Reactome: R-HSA-380270 Reactome: R-HSA-380284 Reactome: R-HSA-380320 Reactome: R-HSA-389957 Reactome: R-HSA-389960 Reactome: R-HSA-389977 Reactome: R-HSA-437239 Reactome: R-HSA-5610787 Reactome: R-HSA-5617833 Reactome: R-HSA-5620912 Reactome: R-HSA-5620924 Reactome: R-HSA-5626467 Reactome: R-HSA-5663220 Reactome: R-HSA-6807878 Reactome: R-HSA-6811434 Reactome: R-HSA-6811436 Reactome: R-HSA-68877 Reactome: R-HSA-8852276 Reactome: R-HSA-8854518 Reactome: R-HSA-8955332 Reactome: R-HSA-9609690 Reactome: R-HSA-9609736 Reactome: R-HSA-9619483 Reactome: R-HSA-9646399 Reactome: R-HSA-9648025 Reactome: R-HSA-9668328 Reactome: R-HSA-983189
Cellular Localisation	Cytoplasm Cytoskeleton
Alternative Antibody Names	Anti-Tubulin Alpha-4a Chain antibody Anti-Alpha-Tubulin 1 antibody Anti-Testis-Specific Alpha-Tubulin antibody Anti-Tubulin H2-Alpha antibody Anti-Tubulin Alpha-1 Chain antibody Anti-TUBA4A antibody Anti-TUBA1 antibody

Information sourced from Uniprot.org

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