• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g Alpha-Catenin antibody (STJ27602). Western blot was performed from the immunoprecipitate using Alpha-Catenin antibody (STJ27602) at a dilution of 1:500.
  • Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded mouse testis using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded human stomach using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded human vermiform appendix using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded rat kidney using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and Alpha-Catenin knockout (KO) 293T cells, using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CTNNA1 antibody (637-906) (STJ27602)

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STJ27602

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Alpha-Catenin (637-906) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTNNA1
Gene ID: 1495
Uniprot ID: CTNA1_HUMAN
Immunogen Region: 637-906
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human Alpha-Catenin (NP_001894.2).
Immunogen Sequence: ELDDSDFETEDFDVRSRTSV QTEDDQLIAGQSARAIMAQL PQEQKAKIAEQVASFQEEKS KLDAEVSKWDDSGNDIIVLA KQMCMIMMEMTDFTRGKGPL KNTSDVISAAKKIAEAGSRM DKLGRTIADHCPDSACKQDL LAYLQRIALYCHQLNICSKV KAEVQNLGGELVVSGVDSAM SLIQAAKNLMNAVVQTVKAS YVASTKYQKSQGMASLNLPA VSWKMKAPEKKPLVKREKQ
Tissue Specificity Expressed ubiquitously in normal tissues.
Post Translational Modifications Sumoylated. Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion.
Function Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. May play a crucial role in cell differentiation.
Protein Name Catenin Alpha-1
Alpha E-Catenin
Cadherin-Associated Protein
Renal Carcinoma Antigen Ny-Ren-13
Database Links Reactome: R-HSA-418990
Reactome: R-HSA-5218920
Reactome: R-HSA-525793
Reactome: R-HSA-5626467
Reactome: R-HSA-9762292
Reactome: R-HSA-9764302
Reactome: R-HSA-9833576
Cellular Localisation Isoform 1: Cytoplasm
Cytoskeleton
Cell Junction
Adherens Junction
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Found At Cell-Cell Boundaries And Probably At Cell-Matrix Boundaries
Isoform 3: Cell Membrane
Alternative Antibody Names Anti-Catenin Alpha-1 antibody
Anti-Alpha E-Catenin antibody
Anti-Cadherin-Associated Protein antibody
Anti-Renal Carcinoma Antigen Ny-Ren-13 antibody
Anti-CTNNA1 antibody

Information sourced from Uniprot.org

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