• Immunohistochemistry analysis of paraffin-embedded human gastric cancer using AK2 antibody (STJ28602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from normal (control) and AK2 knockout (KO) 293T cells, using AK2 antibody (STJ28602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using AK2 antibody (STJ28602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AK2 antibody (1-232) (STJ28602)

SKU:
STJ28602

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AK2 (1-232) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AK2
Gene ID: 204
Uniprot ID: KAD2_HUMAN
Immunogen Region: 1-232
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human AK2 (NP_001616.1).
Immunogen Sequence: MAPSVPAAEPEYPKGIRAVL LGPPGAGKGTQAPRLAENFC VCHLATGDMLRAMVASGSEL GKKLKATMDAGKLVSDEMVV ELIEKNLETPLCKNGFLLDG FPRTVRQAEMLDDLMEKRKE KLDSVIEFSIPDSLLIRRIT GRLIHPKSGRSYHEEFNPPK EPMKDDITGEPLIRRSDDNE KALKIRLQAYHTQTTPLIEY YRKRGIHSAIDASQTPDVVF ASILAAFSKATC
Tissue Specificity Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
Function Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.
Protein Name Adenylate Kinase 2 - Mitochondrial
Ak 2
Atp-Amp Transphosphorylase 2
Atp -Amp Phosphotransferase
Adenylate Monophosphate Kinase Cleaved Into - Adenylate Kinase 2 - Mitochondrial - N-Terminally Processed
Database Links Reactome: R-HSA-499943
Cellular Localisation Mitochondrion Intermembrane Space
Alternative Antibody Names Anti-Adenylate Kinase 2 - Mitochondrial antibody
Anti-Ak 2 antibody
Anti-Atp-Amp Transphosphorylase 2 antibody
Anti-Atp -Amp Phosphotransferase antibody
Anti-Adenylate Monophosphate Kinase Cleaved Into - Adenylate Kinase 2 - Mitochondrial - N-Terminally Processed antibody
Anti-AK2 antibody
Anti-ADK2 antibody

Information sourced from Uniprot.org

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