• Western blot analysis of extracts of various cell lines, using GRK2 antibody (STJ22534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Western blot analysis of extracts from normal (control) and GRK2 knockout (KO) 293T cells, using GRK2 antibody (STJ22534) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using GRK2 Antibody (STJ22534) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using GRK2 Antibody (STJ22534) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using GRK2 Antibody (STJ22534) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-GRK2 antibody (440-689) (STJ22534)

SKU:
STJ22534

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADRBK1 (440-689) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRK2
Gene ID: 156
Uniprot ID: ARBK1_HUMAN
Immunogen Region: 440-689
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human GRK2 (NP_001610.2).
Immunogen Sequence: LGRGAQEVKESPFFRSLDWQ MVFLQKYPPPLIPPRGEVNA ADAFDIGSFDEEDTKGIKLL DSDQELYRNFPLTISERWQQ EVAETVFDTINAETDRLEAR KKAKNKQLGHEEDYALGKDC IMHGYMSKMGNPFLTQWQRR YFYLFPNRLEWRGEGEAPQS LLTMEEIQSVEETQIKERKC LLLKIRGGKQFILQCDSDPE LVQWKKELRDAYREAQQLVQ RVPKMKNKPRSPVVELSKV
Tissue Specificity Expressed in peripheral blood leukocytes.
Function Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity. Inhibits relaxation of airway smooth muscle in response to blue light.
Protein Name Beta-Adrenergic Receptor Kinase 1
Beta-Ark-1
G-Protein Coupled Receptor Kinase 2
Database Links Reactome: R-HSA-111933
Reactome: R-HSA-416476
Reactome: R-HSA-418555
Reactome: R-HSA-5635838
Reactome: R-HSA-8856825
Cellular Localisation Cytoplasm
Cell Membrane
Postsynapse
Presynapse
Alternative Antibody Names Anti-Beta-Adrenergic Receptor Kinase 1 antibody
Anti-Beta-Ark-1 antibody
Anti-G-Protein Coupled Receptor Kinase 2 antibody
Anti-GRK2 antibody
Anti-ADRBK1 antibody
Anti-BARK antibody
Anti-BARK1 antibody

Information sourced from Uniprot.org

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