• Immunohistochemistry analysis of ACTN3 in paraffin-embedded rat skeletal muscle using ACTN3 Rabbit polyclonal antibody (STJ114663) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ACTN3 in paraffin-embedded mouse skeletal muscle using ACTN3 Rabbit polyclonal antibody (STJ114663) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ACTN3 Rabbit polyclonal antibody (STJ114663) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ACTN3 antibody (580-630) (STJ114663)

SKU:
STJ114663

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACTN3 (580-630) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTN3
Gene ID: 89
Uniprot ID: ACTN3_HUMAN
Immunogen Region: 580-630
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 580-630 of human ACTN3 (NP_001095.2).
Immunogen Sequence: GAIMGIQGEIQKICQTYGLR PCSTNPYITLSPQDINTKWD MVRKLVPSCDQ
Tissue Specificity Expressed only in a subset of type 2 skeletal muscle fibers.
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Protein Name Alpha-Actinin-3
Alpha-Actinin Skeletal Muscle Isoform 3
F-Actin Cross-Linking Protein
Database Links Reactome: R-HSA-373753
Reactome: R-HSA-390522
Alternative Antibody Names Anti-Alpha-Actinin-3 antibody
Anti-Alpha-Actinin Skeletal Muscle Isoform 3 antibody
Anti-F-Actin Cross-Linking Protein antibody
Anti-ACTN3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance