• Immunofluorescence analysis of HeLa cells using ACTN2 antibody (STJ22501) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ACTN2 antibody (STJ22501) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACTN2 antibody (1-250) (STJ22501)

SKU:
STJ22501

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACTN2 (1-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTN2
Gene ID: 88
Uniprot ID: ACTN2_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ACTN2 (NP_001094.1).
Immunogen Sequence: MNQIEPGVQYNYVYDEDEYM IQEEEWDRDLLLDPAWEKQQ RKTFTAWCNSHLRKAGTQIE NIEEDFRNGLKLMLLLEVIS GERLPKPDRGKMRFHKIANV NKALDYIASKGVKLVSIGAE EIVDGNVKMTLGMIWTIILR FAIQDISVEETSAKEGLLLW CQRKTAPYRNVNIQNFHTSW KDGLGLCALIHRHRPDLIDY SKLNKDDPIGNINLAMEIAE KHLDIPKMLDAEDIVNTPK
Tissue Specificity Expressed in both skeletal and cardiac muscle.
Post Translational Modifications Ubiquitinated by FBXL22, leading to proteasomal degradation.
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Protein Name Alpha-Actinin-2
Alpha-Actinin Skeletal Muscle Isoform 2
F-Actin Cross-Linking Protein
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-373753
Reactome: R-HSA-390522
Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
Z Line
Colocalizes With Myoz1 And Flnc At The Z-Lines Of Skeletal Muscle
Alternative Antibody Names Anti-Alpha-Actinin-2 antibody
Anti-Alpha-Actinin Skeletal Muscle Isoform 2 antibody
Anti-F-Actin Cross-Linking Protein antibody
Anti-ACTN2 antibody

Information sourced from Uniprot.org

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