• Western blot analysis of lysates from 293, HeLa, and A549 cells, using ACTN1/2/3/4 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of A549 cells using Actinin-Alpha 1/2/3/4 Polyclonal Antibody diluted at 1：1000
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of various cells using Actinin-Alpha 1/2/3/4 Polyclonal Antibody diluted at 1：1000

Anti-Actinin-Alpha 1/2/3/4 antibody (21-70 aa) (STJ91466)

SKU:
STJ91466

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Alpha-actinin-1 and Alpha-actinin-2 and Alpha-actinin-3 and Alpha-actinin-4 (21-70 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTN3
ACTN2
89
88
87
ACTN3_HUMAN
ACTN2_HUMAN
ACTN1_HUMAN
Immunogen Region: 21-70 aa
Specificity: Actinin-Alpha 1/2/3/4 Polyclonal Antibody detects endogenous levels of Actinin-Alpha 1/2/3/4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ACTN1/2/3/4 at the amino acid range 21-70
Protein Name Alpha-Actinin-1
Alpha-Actinin Cytoskeletal Isoform
F-Actin Cross-Linking Protein
Non-Muscle Alpha-Actinin-1
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-3000170
Reactome: R-HSA-373753
Reactome: R-HSA-446388
Cellular Localisation Cytoplasm
Cytoskeleton
Myofibril
Sarcomere
Z Line
Cell Membrane
Cell Junction
Cell Projection
Ruffle
Colocalizes With Myoz2 And Ppp3ca At The Z-Line Of Heart And Skeletal Muscle
Colocalizes With Psd In Membrane Ruffles And Central Reticular Structures
Alternative Antibody Names Anti-Alpha-Actinin-1 antibody
Anti-Alpha-Actinin Cytoskeletal Isoform antibody
Anti-F-Actin Cross-Linking Protein antibody
Anti-Non-Muscle Alpha-Actinin-1 antibody
Anti-ACTN1 antibody

Information sourced from Uniprot.org

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