• Western blot analysis of various cell lines with Actb (beta-Actin) antibody at dilution of 1:2000.
  • Immunohistochemistry of paraffin-embedded human colon cancer with Actb (beta-Actin) antibody at dilution of 1:1000.
  • Immunohistochemistry of paraffin-embedded rat lung with Actb (beta-Actin) antibody at dilution of 1:1000.
  • Immunohistochemistry of paraffin-embedded human lung cancer with Actb (beta-Actin) antibody at dilution of 1:1000.
  • Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using Actb (beta-Actin) antibody at dilution of 1:1000.
  • Immunohistochemistry of paraffin-embedded mouse spleen with Actb (beta-Actin) antibody at dilution of 1:1000.
  • Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using Actb (beta-Actin) antibody at dilution of 1:1000.
Sale

Anti-Actb antibody [SR069J] (STJA0004971)

SKU:
STJA0004971
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody anti-Beta Actin is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: SR069J
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS with 0.02% Sodium Azide, 100 Mu g/mL BSA and 50% Glycerol.
Purification: Affinity purification
Concentration: 0.1 mg/ml
Storage Instruction: Store at-20C for one year. Avoid repeated freeze/thaw cycles.
Gene Symbol: Actb
Gene ID: 11461
Uniprot ID: ACTB_MOUSE
Immunogen: KLH conjugated synthetic peptide corresponding to mouse beta-Actin
Post Translational Modifications ISGylated. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization. Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery. Actin, cytoplasmic 1, N-terminally processed: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
Protein Name Actin - Cytoplasmic 1
Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed
Database Links Reactome: R-MMU-190873
Reactome: -MMU-196025
Reactome: -MMU-2029482
Reactome: -MMU-3928662
Reactome: -MMU-418990
Reactome: -MMU-437239
Reactome: -MMU-4420097
Reactome: -MMU-445095
Reactome: -MMU-446353
Reactome: -MMU-5626467
Reactome: -MMU-5663213
Reactome: -MMU-5663220
Reactome: -MMU-5674135
Reactome: -MMU-8856828
Reactome: -MMU-9013418
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Actin - Cytoplasmic 1 antibody
Anti-Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed antibody
Anti-Actb antibody

Information sourced from Uniprot.org

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