• Western blot analysis of lysates from 1) Hela, 2) Rat brain, 3) Mouse brain cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta Polyclonal Antibody (cat: (STJ96145) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • Western blot analysis of Hela (1), Rat brain (2), Mouse brain (3), (A02) diluted at 1:10000.

Anti-ACTB antibody (C-Term) [A02] (STJ96934)


Current Stock:
Host: Mouse
Applications: WB
Reactivity: Human, Rat, Mouse, Simian, Dog, Chicken, Hamster, Rabbit, Pig, Sheep
Short Description: Mouse monoclonal antibody anti-Actin-Cytoplasmic 1 (C-Term) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: A02
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS, pH 7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was isolated from ascitic fluid by immunoaffinity chromatography using antigens coupled to agarose beads.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTB
Gene ID: 60
Immunogen Region: C-Term
Specificity: ACTB monoclonal antibody (Actin-Cytoplasmic 1) binds to endogenous Actin-Cytoplasmic 1 at the amino acid region C-Term.
Immunogen: Synthetic peptide of Beta-actin
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
Protein Name Actin - Cytoplasmic 1
Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-190873
Reactome: R-HSA-196025
Reactome: R-HSA-2029482
Reactome: R-HSA-3214847
Reactome: R-HSA-389957
Reactome: R-HSA-390450
Reactome: R-HSA-3928662
Reactome: R-HSA-3928665
Reactome: R-HSA-418990
Reactome: R-HSA-437239
Reactome: R-HSA-4420097
Reactome: R-HSA-445095
Reactome: R-HSA-446353
Reactome: R-HSA-5250924
Reactome: R-HSA-5626467
Reactome: R-HSA-5663213
Reactome: R-HSA-5663220
Reactome: R-HSA-5674135
Reactome: R-HSA-5689603
Reactome: R-HSA-5696394
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-8856828
Reactome: R-HSA-9035034
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Reactome: R-HSA-9664422
Reactome: R-HSA-983231
Cellular Localisation Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Actin - Cytoplasmic 1 antibody
Anti-Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed antibody
Anti-ACTB antibody

Information sourced from Uniprot.org

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