• Western blot detection of beta actin in Jurkat, K562, C6, 3T3 and Hela cell lysates using beta actin mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.

Anti-ACTB antibody [8F10-G10] (STJ99029)


Current Stock:
Host: Mouse
Applications: WB
Reactivity: Human, Mouse, Rat
Short Description: Mouse monoclonal antibody anti-Actin-Cytoplasmic 1 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: 8F10-G10
Conjugation: Unconjugated
Isotype: IgG2b
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was isolated from ascitic fluid by immunoaffinity chromatography using antigens coupled to agarose beads.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTB
Gene ID: 60
Specificity: ACTB monoclonal antibody (Actin-Cytoplasmic 1) binds to endogenous Actin-Cytoplasmic 1.
Immunogen: Purified recombinant human beta actin protein fragments expressed in E.coli.
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
Protein Name Actin - Cytoplasmic 1
Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-190873
Reactome: R-HSA-196025
Reactome: R-HSA-2029482
Reactome: R-HSA-3214847
Reactome: R-HSA-389957
Reactome: R-HSA-390450
Reactome: R-HSA-3928662
Reactome: R-HSA-3928665
Reactome: R-HSA-418990
Reactome: R-HSA-437239
Reactome: R-HSA-4420097
Reactome: R-HSA-445095
Reactome: R-HSA-446353
Reactome: R-HSA-5250924
Reactome: R-HSA-5626467
Reactome: R-HSA-5663213
Reactome: R-HSA-5663220
Reactome: R-HSA-5674135
Reactome: R-HSA-5689603
Reactome: R-HSA-5696394
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-8856828
Reactome: R-HSA-9035034
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Reactome: R-HSA-9664422
Reactome: R-HSA-983231
Cellular Localisation Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Actin - Cytoplasmic 1 antibody
Anti-Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed antibody
Anti-ACTB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance