• Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain using Alpha-SMA monoclonal antibody.
  • Immunohistochemical analysis of paraffin-embedded Mouse Cecal Tissue using Alpha-SMA monoclonal antibody.
  • Immunofluorescence analysis of Mouse-liver tissue. 1, Alpha-SMA monoclonal antibody (6A12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Rat-liver tissue. 1, Alpha-SMA monoclonal antibody (6A12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Human-liver tissue. 1, Alpha-SMA monoclonal antibody (6A12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Alpha-SMA monoclonal antibody (6A12) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, Alpha-SMA monoclonal antibody (6A12) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, Alpha-SMA monoclonal antibody (6A12) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of Hela cell. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-SMA monoclonal antibody (6A12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ACTA2 antibody [6A12] (STJ97382)

SKU:
STJ97382

£42.00 - £189.00
Current Stock:
Host: Mouse
Applications: WB, IF, ICC, IHC-P
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Mouse monoclonal antibody anti-Actin-Aortic Smooth Muscle is suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 6A12
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS, pH 7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was isolated from ascitic fluid by immunoaffinity chromatography using antigens coupled to agarose beads.
Dilution Range: WB 1:10000-100000
IF 1:200
IHC 1:200-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTA2
Gene ID: 59
Uniprot ID: ACTA_HUMAN
Specificity: ACTA2 monoclonal antibody (Actin-Aortic Smooth Muscle) binds to endogenous Actin-Aortic Smooth Muscle.
Immunogen: Synthetic peptide of Alpha-SMA
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Protein Name Actin - Aortic Smooth Muscle
Alpha-Actin-2
Cell Growth-Inhibiting Gene 46 Protein Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form
Database Links Reactome: R-HSA-445355
Reactome: R-HSA-9013695
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Actin - Aortic Smooth Muscle antibody
Anti-Alpha-Actin-2 antibody
Anti-Cell Growth-Inhibiting Gene 46 Protein Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form antibody
Anti-ACTA2 antibody
Anti-ACTSA antibody
Anti-ACTVS antibody
Anti-GIG46 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance