• Western blot analysis of extracts from various cell lines, using ACLY rabbit monoclonal antibody (STJ11101939) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Confocal imaging of NIH/3T3 cells using ACLY rabbit monoclonal antibody (STJ11101939, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Confocal imaging of U-2 OS cells using ACLY rabbit monoclonal antibody (STJ11101939, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunoprecipitation analysis of 300 Mu g extracts from HepG2 cells using 3 Mu g ACLY antibody (STJ11101939). Western blot was performed from the immunoprecipitate using ACLY antibody (STJ11101939) at a dilution of 1:1000.

Anti-ACLY antibody (1000-1101) [S9MR] (STJ11101939)

SKU:
STJ11101939

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ACLY (1000-1101) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACLY
Gene ID: 47
Uniprot ID: ACLY_HUMAN
Immunogen Region: 1000-1101
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1000-1101 of human ACLY (P53396).
Immunogen Sequence: ATPLLDYALEVEKITTSKKP NLILNVDGLIGVAFVDMLRN CGSFTREEADEYIDIGALNG IFVLGRSMGFIGHYLDQKRL KQGLYRHPWDDISYVLPEHM SM
Post Translational Modifications Phosphorylated by PKA and GSK3 in a sequential manner.phosphorylation results in activation of its activity. Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455. Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues. ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by the BCR(KLHL25) E3 ubiquitin ligase complex and UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site. BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells.
Function Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.
Protein Name Atp-Citrate Synthase
Atp-Citrate
Pro-S--Lyase
Acl
Citrate Cleavage Enzyme
Database Links Reactome: R-HSA-163765
Reactome: R-HSA-6798695
Reactome: R-HSA-75105
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Atp-Citrate Synthase antibody
Anti-Atp-Citrate antibody
Anti-Pro-S--Lyase antibody
Anti-Acl antibody
Anti-Citrate Cleavage Enzyme antibody
Anti-ACLY antibody

Information sourced from Uniprot.org

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